Porcine embryonic stem cells forming germ line chimeras

猪胚胎干细胞形成种系嵌合体

• 批准号: 07506005
• 负责人: SATO Eimei
• 金额: $ 9.92万
• 依托单位: Faculty of Agriculture, Tohoku University (1997)The University of Tokyo (1995-1996)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07506005/
• 关键词: germ line; germ lne chimera; primordial germ cell; chimera; LIF; embryonic stem cell; pig; xenotransplantation; 血血病抑制因子; 異種移植

1) Porcine 1-cell embryos were produced by the conventional or the new in vitro maturation/fertilization system and cultured in NCSU-23 supplemented with hyaluronic acid at various concentrations. Our results indicate that effect of hysluronic acid on development of in vitro produced porcine embryos varies with the conditions of maturation/fertilization of the oocytes.2) Porcine blastocysts were produced from immature oocytes by using _iVMFC,and cultured on STO feeder layrs to determine whether they can be used for studies on establishment of ES cells. when hatched blastocysts that had been obtained by culturing the intact blastocysts in the medium supplemented with FCS were transferred, they attached to the feeder cells. The results of the present study suggest that in vitro produced porcine blastocysts can be used for studies on establishment of ES cells.3) Our results demonstrated that single ES cells which maintain totipotency or pluripotency of high degree are present in a colony of ES cells, and that single ES cells cojoined with the blastomeres of 8-cell-stage embryos may colonize, if the circumustances allow, almost exclusively the germ cells and concomitantly the urogenital cell lineages.

1）猪1细胞胚胎是通过常规或新的体外成熟/受精系统产生的，并在补充各种浓度的透明质酸的NCSU-23中培养。我们的结果表明，息护酸对体外产生的猪胚胎发育的影响随卵母细胞的成熟/受精的条件而变化。2）使用_IVMFC通过_IVMFC产生未成熟的卵母细胞的猪囊肿，并确定是否在Sto Fifeer Layr上培养。它们可用于建立ES细胞的研究。当孵化的胚泡通过在补充FC的培养基中培养完整的胚泡获得的胚泡时，它们附着在进料器细胞上。本研究的结果表明，体外产生的猪胚泡可用于建立ES细胞的研究。3）我们的结果表明，在ES细胞的一个群体中，存在维持高素质或高度多能性的单个ES细胞，并且，如果繁殖允许，几乎完全是生殖细胞和泌尿生殖器细胞谱系的单个ES细胞与8细胞阶段胚胎的胚泡可能会定植。

项目成果

佐藤英明: "新農学シリーズ 家畜繁殖(分担)" 朝倉書店, 173 (1995)

佐藤秀明：《新农科系列畜牧育种（分享）》朝仓书店173（1995）

佐藤英明: "胚様S本を形成する細胞群の性状とその多分化能" 日本胚移植学雑誌. 3. 印刷中 (1996)

Hideaki Sato：“形成胚胎样 S-book 的细胞群的特征及其多能性”，日本胚胎移植杂志 3。出版中（1996 年）。

佐藤 英明: "Hyaluronic acid-binding proteins in porcine cumulus cells:involvement with cumulus expansion" Biology of Reproduction. 58(印刷中). (1998)

Hideaki Sato：“猪卵丘细胞中的透明质酸结合蛋白：与卵丘扩张有关”《生殖生物学》58（出版中）。

佐藤英明: "クローン羊" サイアス. 12. 72-73 (1997)

佐藤秀明：“克隆羊”赛亚斯。12. 72-73 (1997)

内藤邦彦: "Effects of vanadate on meiotic maturation of porcine oocytes in vitro" Journal of Reproduction and Development. 42(印刷中). (1996)

Kunihiko Naito：“钒酸盐对体外猪卵母细胞减数分裂的影响”，《生殖与发育杂志》42（出版中）。