Effects of various drugs on pancreatic exocrine function in cerulein-induced acute pancreatitis in the rat

不同药物对雨蛙素诱发急性胰腺炎大鼠胰腺外分泌功能的影响

• 批准号: 07671425
• 负责人: SUGIYAMA Masanori
• 金额: $ 1.41万
• 依托单位: Kyorin University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07671425/
• 关键词: acute pancreatitis; pancreatic exocrine function; cerulein; secretin

We experimentally investigated effect of secretin on pancreatic exocrine function in cerulein-induced acute pancreatitis in the rat. Cholecystokinin-stimulated pancreatic secretion was examined in conscious rats after surgical preparation with external pancreatic fistula. Four days after surgical preparation, we induced acute pancreatitis by intravenous administration of cerulein at a rate of 10 mug/kg/h for 4 hours, followed by administration of normal saline (control group) or secretin (5CU/kg/h, 8 hours ; secretin group). Pancreatic exocrine function test was undertaken one day before and after cerulein administration. Microscopically, the pancreas in control group showed prominent interstitial edema and intracellular vacuolization of the acinar cell. These findings were partially improved after secretin administration. In control group, basal and cholecystokinin-stimulated pancreatic juice flow, bicarbonate output, and protein output decreased significantly after cerulein administration. Secretin reestablished pancreatic juice flow and bicarbonate output, and partially restored protein output. In rats with cerulein-induced pancreatitis, secretin reduced pancreatic histopathology and restored exocrine dysfunction, when compared with non-secretin-treated rats.

我们通过实验研究了胰泌素对雨蛙素诱导的大鼠急性胰腺炎胰腺外分泌功能的影响。在进行胰外瘘手术准备后，在清醒的大鼠中检查胆囊收缩素刺激的胰腺分泌。手术准备四天后，我们以10μg/kg/h的速度静脉注射雨蛙素4小时，然后注射生理盐水（对照组）或促胰液素（5CU/kg/h，8小时）诱发急性胰腺炎。 ; 促胰液素组）。在雨蛙素给药前一天和给药后一天进行胰腺外分泌功能测试。镜下观察，对照组胰腺间质水肿明显，腺泡细胞胞内空泡化。施用促胰液素后，这些结果得到部分改善。在对照组中，给予雨蛙素后，基础和胆囊收缩素刺激的胰液流量、碳酸氢盐输出和蛋白质输出显着降低。促胰液素重建胰液流量和碳酸氢盐输出，并部分恢复蛋白质输出。在患有雨蛙素诱导的胰腺炎的大鼠中，与未接受促胰液素治疗的大鼠相比，促胰液素可减轻胰腺组织病理学并恢复外分泌功能障碍。