Immunohistochemical localization of beta1,4-galactosyltransferase in human normal and neoplastic pancreatic tissues

人类正常和肿瘤性胰腺组织中 β1,4-半乳糖基转移酶的免疫组织化学定位

• 批准号: 09671335
• 负责人: SUGIYAMA Masanori
• 金额: $ 1.15万
• 依托单位: Kyorin University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09671335/
• 关键词: galactosyltransferase; pancreatic neoplasm; immunohistochemistry

beta1, 4-galactosyltransferase (GalTase) is the glycosyltransferase in the Golgi apparatus that transfers galactose from UDP-galactose to terminal N-acetylglucosamine residues in glycoconjugates with formation of beta1,4 linkage. Neoplasms undergo various changes in the carbohydrate moieties of their glycosyltransferase themselves. This process also indicates the possibility of changes in glycosyltransferase themselves. Therefore, we examined the binding pattern of a monoclonal antibody (MAb8628) against GalTase in both normal and neoplastic exocrine tissues. Ten normal and II neoplastic human exocrine pancreatic tissues obtained from surgical operations were used. Frozen sections were incubated with this antibody. Supra-nuclear regions and terminal bars of normal duct cells and acinar cells revealed positive staining for GalTase at the light microscopic level. Centroacinar cells revealed positive staining in their pen-nuclear region. Neoplastic cells were also stained in their supra-nuclear regions and terminal bars. Supra-nuclear regions were well developed in neoplastic cells and intensely stained compared with those in normal cells. The supra-nuclear regions and the terminal bars corresponded to the trans cistern of the Golgi apparatus and the junctional complex (i. e. , tight junction and adherens junction), respectively, seen at the electron microscopic level. Pancreatic neoplastic changes thus lead to an increase in the expression of GalTase in the Golgi apparatus, which increase may have an important effect on the intercelluar adhesion and communication among pancreatic epithelial cells. Measurement of this enzyme is useful for diagnois of exocnine pancreatic neoplastic change from normal tissues.

Beta1，4-半乳糖基转移酶（Galtase）是高尔基体中的糖基转移酶，它将半乳糖从UDP-半乳糖转移到末端N-乙酰葡萄糖胺在糖果糖中的末端N-乙酰葡萄糖残基，并形成β1,4链接。肿瘤会在其糖基转移酶本身的碳水化合物部分发生各种变化。该过程还表明了糖基转移酶本身会改变的可能性。因此，我们检查了正常和肿瘤外分泌组织中单克隆抗体（MAB8628）与Galtase的结合模式。使用了10种正常和II肿瘤的人外分胰腺组织，从手术手术中获得。将冷冻切片与该抗体一起孵育。正常导管细胞和腺泡细胞的核核区域和末端条揭示了在光显微镜水平上的galtase阳性染色。质心细胞在其笔核区域显示阳性染色。肿瘤细胞还染色在其上核区域和末端棒中。与正常细胞相比，肿瘤细胞中的核区域在肿瘤细胞中发育良好，并染色。上核区域和末端条分别对应于高尔基体和连接络合物（即，紧密连接和粘附连接）的反式水箱，在电子显微镜水平上可见。因此，胰腺肿瘤的变化导致高尔基体中Galtase表达的增加，这可能会对胰腺上皮细胞之间的界面粘附和通讯具有重要影响。该酶的测量可用于正常组织中外胞菌素胰腺肿瘤变化的诊断。