Effects of chronic pancreatic impairment on development of acute pancreatitis

慢性胰腺损伤对急性胰腺炎发展的影响

• 批准号: 11671270
• 负责人: SUGIYAMA Masanori
• 金额: $ 1.54万
• 依托单位: Kyorin University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11671270/
• 关键词: bacterial translocation; acute pancreatitits; chronic pancreatitis; microbial translocetion; 糖尿病; 感染症; bacterial translocation; acute pancreatitits; chronic pancreatitis; microbial translocetion; 糖尿病; 感染症

Little is known about pancreatic exocrine function during acute exacerbation in patients with chronic pancreatitis. We investigated changes in pancreatic exocrine function after inducing acute pancreatitis in an animal model of spontaneous chronic pancreatitis. WBN/Kob rats with chronic pancreatitis sequentially underwent pancreatic exocrine function test one to six days after surgical preparation with external pancreatic fistula. We induced acute pancreatitis in another WBN/Kob rats by intravenous administration of caerulein at a rate of 10 μg/kg/hr for 4 hours four days after surgical preparation. Pancreatic exocrine function test was undertaken in a conscious state one day before and after caerulein administration. In WBN/Kob rats not given caerulein, pancreatic exocrine function remained almost constant at 3-6 days after surgery. Marked hyperamylasemia developed immediately after caerulein administration. After its administration, the pancreas microscopically showed prominent interstitial edema and intracellular vacuolization of acinar cells in addition to the finding of preexisting chronic pancreatitis. Basal and cholecystokinin-stimulated flow rate, bicarbonate output, and protein output, which were substantially impaired one day before caerulein administration, were further reduced one day after its administration. Pancreatic exocrine hypofunction is markedly deteriorated during acute exacerbation in a rat model with chronic pancreatitis.

关于慢性胰腺炎患者急性加重期间胰腺外分泌功能知之甚少。我们研究了在赞助慢性胰腺炎的动物模型中诱导急性胰腺炎后胰腺外分泌功能的变化。慢性胰腺炎的WBN/KOB大鼠顺序进行了胰腺外分泌功能测试，手术后一到六天用外部胰腺瘘管进行了一到六天。我们通过静脉内服用Caerulein以10μg/kg/kg/hr的速率诱导另一位WBN/KOB大鼠急性胰腺炎，在手术准备后四天4小时。胰蛋白酶给药前后，在有意识的状态下进行了胰腺外分泌功能测试。在未给予囊蛋白的WBN/KOB大鼠中，胰腺外分泌功能在手术后3-6天保持恒定。高氨基血症在给药后立即发展，除了发现先前存在的慢性胰腺炎外，胰腺显着显示了明显的间质水肿和腺泡细胞内的细胞内液泡。在给药前一天，在塞拉林给药前一天，基础和胆囊动蛋白刺激的流速，碳酸氢盐输出和蛋白质产量显着受损。在患有慢性胰腺炎的大鼠模型中，在急性加重过程中明显检测到胰腺外分泌功能障碍。