Studies on the role of the serotonergic neuron system in the plasticity of the developing brain

血清素能神经元系统在大脑发育可塑性中的作用研究

基本信息

批准号：
07457181
负责人：
TAKEUCHI Yoshihiro
金额：
$ 2.37万
依托单位：
Kyoto Prefectural University of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07457181/
关键词：
Serotonin Dopamine Plasticity Sprouting Histochemistry 細胞化学

项目摘要

Serotonin (5-HT) is related to the plasticity of the brain, and 5-HT heterotypic sprouting occurs in the neostriatum of rats that suffer 6-hydroxydopamine (6-OHDA) lesioning in the neonatal period. 6-OHDA was administered to the right lateral ventricle of animals (rat and mouse) at 3 days, 7 days, 12 days and 28 days after birth. Then the animals were perfused transcardially at 2 days, 5 weeks, 10 weeks and 1 year after the administration of 6-OHDA.Immunohistochemical studies were performed with anti-tyrosine hydroxylase (TH) and anti-5-HT antibodies. NADPH-diaphorase histochemistry was also performed. Quantitative analysis on NADPH-positive cells and fiber terminals were performed using NIH Image at three coronal levels.This study has shown that remarkable hyperinnervation of serotonin fibers occurred in the striatum of mice that had undergene the same kind of unilateral 6-OHDA lesioning at even 12 days after birth. This is an important finding as to the critical period for serotonergic heterotypic sprouting after dopamine denervation. Noteworthy is that intrinsic serotonergic heterotypic sprouting persisted throughout the time course of the study, namely, to one year after 6-OHDA lesioning, suggesting that the sprouting is long lasting. There is no significant change in the density of NADPH-positive structures in the striatum during the development of serotonergic heterotypic sprouting after dopamine-depletion. This is the first demonstration that 5-HT hyperinnervation occurs in the neostriatum of mice suffering 6-OHDA lesioning at even 12 days after birth, and that 5-HT heterotypic sprouting may be permanent.

5-羟色胺（5-HT）与大脑的可塑性有关，5-HT异型发芽发生在新生儿时期患有6-羟基多巴胺（6-OHDA）病变的大鼠的新纹状体中。在出生后的3天，7天，12天和28天，将6-OHDA用于动物（大鼠和小鼠）的右侧心室（大鼠和小鼠）。然后在服用6-OHDA后2天，5周，10周和1年对动物进行灌注，并用抗酪氨酸羟化酶（TH）和抗5-HT抗体进行免疫组织化学研究。还进行了NADPH-肌酶组织化学。对NADPH阳性细胞和纤维末端的定量分析使用NIH图像在三个冠状动脉水平上进行。这项研究表明，在出生后12天后，在同样的单侧6-OHDA病变的小鼠的纹状体中发生了5-羟色胺纤维的显着过度感染。这是关于多巴胺神经胺后血清素能异型发芽的关键时期的重要发现。值得注意的是，在整个研究过程中，固有的血清素能异型发芽持续存在，即6-OHDA病变后一年，这表明发芽持续了持久。在多巴胺 - 脱离后血清素能异型发芽期间，纹状体中NADPH阳性结构的密度没有显着变化。这是第一次证明5-HT过度连接发生在出生后12天后患有6-OHDA病变的小鼠的新纹状体中，并且5-HT异型发芽可能是永久性的。