HISTOCHEMICAL STUDIES ON TRANSMITTER-RELATED NEURON SYSTEM UNDER EXPERIMENTALLY INDUCED SEIZURES

实验诱发癫痫发作下递质相关神经元系统的组织化学研究

基本信息

批准号：
63570452
负责人：
TAKEUCHI Yoshihiro
金额：
$ 1.28万
依托单位：
KYOTO PREFECTURAL UNIVERSITY OF MEDICINE
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1988
资助国家：
日本
起止时间：
1988 至 1989
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63570452/
关键词：
Seizure Serotonin Mouse Neostriatum Immunohistochemistry 痙攣

项目摘要

Serotonin neurons in the brainstem give rise to highly branched fibers which innervate virtually the entire central nervous system, comprising the most expatisive neuronal network among other neurotransmitters. The aim of this project is to examine changes occurring in the serotonin neuron system in experimentally induced convulsions by means of a modified immunoperoxidase method. To obtain quantitative data on the density or serotonergic innervation, the total length of serotonin-immunoreactive fibers was calculated using the MOP system. A marked increase in serotonin immunoreactivity was observed at the rostral and intermediate levels or the neostriatum of mice kept at high temperature (50ﾟC) with no convulsions, and a significant reduction in serotonin immunoreactivity was verified throughout the neostriatum of mice which had hyperthermia-induced seizures. in the case or pilocarpine-induced convulsions, a significant reduction in serotonin immunoreactivity was observed at the rostral and caudal levels of the neostriatum or mice which had generalized seizures due to the systemic administration of pilocarpine (350 mg/kg). In both experimental convulsion models, the serotonin immunoreactivity remained unchanged in the paleostriatum and neocortex and the degree of serotonin reduction in the neostriatum was significantly greater in hyperthermia-induced convulsions than that in pilocarpine-induced convulsions. These results suggest that striatal serotonin may be an important mediater in the mechanism of convulsions and that more precise regional analysis using quantitative histochemical methods is essential for elucidation of the mechanism underlying human neurological disorders.

脑干中的5-羟色胺神经元产生了高度分支的纤维，这些纤维几乎支配了整个中枢神经系统，从而在其他神经递质中完成了最远见的神经元网络。该项目的目的是检查通过改良的免疫过氧化物酶方法，在实验诱导的抽搐中发生5-羟色胺神经元系统中发生的变化。为了获得有关密度或血清素能神经的定量数据，使用MOP系统计算了5-羟色胺免疫反应纤维的总长度。在没有抽搐的高温（50ﾟC）的小鼠（50°C）的小鼠的胸膜和中等水平或新生层面上观察到了5-羟色胺免疫反应性的显着增加，并且在整个患有超疗法诱导的持续性的小鼠的整个小鼠的整个小鼠中，羟色胺免疫反应性的显着降低。在该病例或毛car虫引起的抽搐中，由于全身性癫痫发作，由于全身性癫痫发作（350 mg/kg），新的和小鼠的尾骨和尾部水平显着降低了5-羟色胺免疫反应性。在这两个实验性抽搐模型中，在古纹状体和新皮层中，5-羟色胺免疫反应性保持不变，而在高热诱导的抽搐中，新纹状体中的5-羟色胺还原程度明显大于毛磷脂诱导的脉冲脉冲。这些结果表明，纹状体5-羟色胺可能是抽搐机制的重要媒介，并且使用定量组织化学方法更精确的区域分析对于阐明人类神经系统疾病的基本机制至关重要。