Studies on the role of the serotonin and NMDA receptors in the plasticity of the developing brain

血清素和 NMDA 受体在大脑发育可塑性中作用的研究

基本信息

批准号：
09470184
负责人：
TAKEUCHI Yoshihiro
金额：
$ 2.75万
依托单位：
Kyoto Prefectural University of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09470184/
关键词：
Serotonin NMDA receptor Nitric oxide thiamine-deficiency Plasticity NADPH-α

项目摘要

Distributions of nitric oxide synthase (NOS) neurons and serotonin (5-hidroxytyptamine, 5-HT) were compared throughout the mouse brain using NADPH-d histochemistry and 5-HT immunohistochemistry. The areas where NADPH-diaphorase (NADPH-d) and 5-HT positive cell bodies and fibers were much concentrated together included the medial part of the anterior olfactory nucleus, the medial septal nucleus, the diagonal band of Broca, the medial forebrain bundle, the supraependymal plexus of the ventricles, the ventrolateral geniculate nucleus, the dorsomedial subnucleus of the interpeduncular nucleus, the raphe magnus nucleus, the raphe obscurus nucleus, the dorsal raphe nucleus, the ambiguus nucleus, the solitary tract nucleus and the dorsal motor nucleus of the vagus.We examined changes in 5-HT neurons in pyrithiamine-induced thiamine deficiency in mice immunohistochemically. Extensive decreases in the densities of 5-HT-immunoreactive fibers were detected in the lateral septal nucleus, the thala … More mus, the medial mammillary nucleus, the dorsal and the median raphe nuclei, the raphe obscurus nucleus, the tegmental area, the cerebellum and the vestibular nucleus, though only a small decrease was detected in the inferior colliculus. Most remarkably, degenerative winding fibers were detected between the deep mecencephalic nucleus and the ventral tegmental area. Increases in intensity of 5-HT immunoreactivity in the dorsal raphe nucleus and decreases in the number of 5-HT-immunoreactive cell bodies in the dorsal and the median raphe nuclei were detected. These results suggest the differential vulnerability of 5-HT neurons in thiamine-deficient mice.Changes in NOS neurons in thiamine-deficient mice were also examined histochemically using NADPH-d, an identical marker for NOS containing neurons. Decreases in the number of cell bodies and the densities of fibers in NADPH-d- containing neurons were detected in particular areas of the thalamus, the lateral habenular nucleus, the dorsal raphe nucleus and the medial vestibular nucleus, and decreases in the density of the fibers was detected also in the caudal part of the medial portion of the medial mammillary nucleus in thiamine-deficient mice. A marked loss of NADPH-d-containing neurons was also detected in the hippocampus. The results suggest the possibility that the influence of NOS includes some neuropathological vulnerability of the brain to NOS in experimental thiamine deficiency. Less

使用NADPH-D组织化学和5-HT免疫组织化学比较了整个小鼠脑的一氧化氮合酶（NOS）神经元和5-羟基苯丙胺，5-HT的分布。 The areas where NADPH-diaphorase (NADPH-d) and 5-HT positive cell bodies and fibers were much concentrated together included the media part of the anterior olfactory nuclearus, the media septal nucleus, the diagonal band of Broca, the media Forebrain bundle, the supraependymal plexus of the ventricles, the ventrolateral geniculate nucleus, the dorsomedial subnucleus of枝间核，raphe magnus nuc，raphe submurous核，背部raphe核，歧义核，核核，固体道核和传奇的背运动核。我们检查了吡啶胺诱导的硫胺素诱导的硫胺素诱导的5-HT神经元的变化，硫胺素诱导的硫胺素诱导的硫胺素缺乏症。在隔膜核核，塔拉（Thala），更多的MUS，MUS，媒体乳腺核心，背侧和中间的Raphe Nougus，Raphe cookurus unitus，te术区域，cerebellum unitorus contrioruul中，发现了较小的核心，但在较小的核心中发现了5-HT免疫反应纤维的大量下降。最引人注目的是，在深脑核心和腹侧段区域之间检测到退化性绕组纤维。背侧raphe核的5-HT免疫反应性的强度增加，并且检测到背侧5-HT免疫反应性细胞体的数量下降。这些结果表明，还使用NADPH-D进行了组织化学研究，这是硫胺素缺陷小鼠的NOS神经元中5-HT神经元的差异脆弱性。在丘脑的特定区域，外侧核心核和背侧raphe核和培养基核核和培养基核核的纤维密度下，在培养基的密度上增加了媒体的媒体虫虫，在培养基的密度上也增加了细胞体的数量和含有NADPH-D的神经元的纤维密度的减少。在海马中还检测到了明显的含NADPH-D的神经元的损失。结果表明，NOS的影响包括大脑在实验性硫胺素缺乏症中NOS的一些神经病理学脆弱性。较少的