The mechanism of neurotransmitter release and its control using electric organ synapse of electric ray

电射线电器官突触释放神经递质及其控制机制

基本信息

批准号：
06404076
负责人：
KIRINO Yutaka
金额：
$ 17.86万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (A)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

项目摘要

1. cDNA cloning of voltage-dependent Ca channel of electromotor neuron from electric ray.RT-PCR was carried out with electric lobe of Japanese electric ray, Narke Japonica. Partial sequence of N- and L-type voltage dependent Ca channel were obtained.2. Experimental setup to investigate the electrophysiological characteristics of Ca channel of electric organ nerve terminal.In order to do the electrophysiological characterization of Ca channel of electric organ synaptosomes, lipid bilayer system was introduced.Preliminary experiments revealed that a voltage dependent anion channel was detected in ciliary membrane vesicles from Teterahymena.3. Asymmetrical distribution of phospholipids in membrane of synaptosome and synaptic vesicle from electric ray.The asymmetrical distribution of phospholipids between the outer and inner leaflets of synaptosome and synaptic vesicle was investigated. In both membranes, phosphatidylserine (PS) was dominant in the leaflet which faces cytoplasm.4. The effects of adenosine on the acetylcholine release from electric organ synaptosomes.The effects of adenosine on the acetylcholine (ACh) release from synaptosomes was investigated. Adenosine inhibited the ACh release through adenosine Al receptor and facilitated the release through A2 receptor. The inhibitory effect via Al receptor was caused by the inhibition of N-type voltage dependent Ca channel, and the facilitatory effects via A2 receptor was caused by the activation of P-type Ca channel.

1。电动射线电动神经元电压依赖性Ca通道的cDNA克隆。rt-pcr用日本电动射线的电叶Narke Japonica进行。获得了N型电压依赖性Ca通道的部分序列2。实验设置为了研究电器官神经终端CA通道的电生理特性。为了进行电机器官突触体CA通道的电生理表征，引入了脂质双层系统。脂质双层系统被引入。培训表明，在纤毛膜触发器中检测到电压依赖性阴离子通道。对突触体和突触囊泡的磷脂的不对称分布研究了突触体和突触囊泡外部和内部小叶之间的磷脂的不对称分布。在这两个膜中，磷脂酰丝氨酸（PS）在面对细胞植物的传单中占主导地位。4。研究了腺苷对乙酰胆碱从电器官突触体释放的影响。腺苷对乙酰胆碱（ACH）从突触体释放的影响。腺苷通过腺苷Al受体抑制ACH释放，并通过A2受体促进释放。通过AL受体的抑制作用是由N型电压依赖性CA通道的抑制作用引起的，并且通过A2受体的促进作用是由P型Ca通道的激活引起的。