Analysis of memory formation and readout in the slug

slug中的记忆形成和读出分析

基本信息

批准号：
12307053
负责人：
KIRINO Yutaka
金额：
$ 26.3万
依托单位：
THE UNIVERSITY OF TOKYO
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2002
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12307053/
关键词：
slug associative learning procerebrum nonbursting neuron inhibitory postsynaptic potential in vitro learning burst dischanges nystatin perforated patch recording 遺伝子導入長期記憶遺伝子発現 differential display RNAi 神経修飾物質タンパク合成

项目摘要

Slugs perform odor-taste associative learning, and the present work was aimed at clarifying the mechanisms of odor learning using physiological and molecular biological methods. A high frequency stimulation of the lip nerve, which is an in vitro analog of the unconditioned stimulus, caused an increased frequency in the local Meld potential oscillation in the procerebrum (PC), as well as excitation of the bursting PC neurons and inhibition of the nonbursting PC neurons. As the nonbursting neurons are thought to be the principal neurons of the PC and may mediate output of information from the PC to other brain regions, these results suggest that output of information from the PC is decreased during conditioning. A high frequency stimulation of the tentacle nerve resulted in a potentiation of the evoked potential in the PC, which lasted for more than 2 h. Such plastic changes in the efficacy of synaptic transmission in the PC may be involved in odor learning.Injection of the protein synthesis inhibitors anisomycin or cycloheximide impaired memory retention after 2-7 days alter conditioning, indicating that protein synthesis is necessary for the retention of long-term memory. We further explored genes induced by the odor-taste associative conditioning using the PCR-differential display method in the brains from paired and unpaired conditioned animals. Using 100 pairs of random primers, 12 bands showed a reproducible induction pattern, and partial sequences of 8 genes were cloned.

SLUGS进行气味泥关系的关联学习，目前的工作旨在阐明使用生理和分子生物学方法的气味学习机制。唇神经的高频刺激是无条件刺激的体外类似物，导致局部MELD电位振荡的频率增加（PC）（PC），以及对爆发的PC神经元的激发和对非爆炸PC神经元的抑制。由于非爆炸神经元被认为是PC的主要神经元，并且可能会介导PC的信息输出到其他大脑区域，因此这些结果表明，调节过程中，来自PC的信息的输出减少了。触手神经的高频刺激导致PC中诱发电位的增强，该电位持续了2小时以上。这种塑性在PC中的突触传播功效的变化可能与气味学习有关。蛋白质合成抑制型砷霉素或环己酰亚胺在2-7天后进行调节后保留记忆力保留，这表明蛋白质合成是长期记忆的保留所必需的。我们进一步探索了由配对和未配对的动物的大脑中的PCR-差异显示方法，由气味泥关系缔合条件诱导的基因。使用100对随机引物，12个带显示了可再现的诱导模式，并克隆了8个基因的部分序列。