Analysis of memory formation and readout in the slug

slug中的记忆形成和读出分析

基本信息

批准号：
12307053
负责人：
KIRINO Yutaka
金额：
$ 26.3万
依托单位：
THE UNIVERSITY OF TOKYO
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2002
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12307053/
关键词：
slug associative learning procerebrum nonbursting neuron inhibitory postsynaptic potential in vitro learning burst dischanges nystatin perforated patch recording 遺伝子導入長期記憶遺伝子発現 differential display RNAi 神経修飾物質タンパク合成

项目摘要

Slugs perform odor-taste associative learning, and the present work was aimed at clarifying the mechanisms of odor learning using physiological and molecular biological methods. A high frequency stimulation of the lip nerve, which is an in vitro analog of the unconditioned stimulus, caused an increased frequency in the local Meld potential oscillation in the procerebrum (PC), as well as excitation of the bursting PC neurons and inhibition of the nonbursting PC neurons. As the nonbursting neurons are thought to be the principal neurons of the PC and may mediate output of information from the PC to other brain regions, these results suggest that output of information from the PC is decreased during conditioning. A high frequency stimulation of the tentacle nerve resulted in a potentiation of the evoked potential in the PC, which lasted for more than 2 h. Such plastic changes in the efficacy of synaptic transmission in the PC may be involved in odor learning.Injection of the protein synthesis inhibitors anisomycin or cycloheximide impaired memory retention after 2-7 days alter conditioning, indicating that protein synthesis is necessary for the retention of long-term memory. We further explored genes induced by the odor-taste associative conditioning using the PCR-differential display method in the brains from paired and unpaired conditioned animals. Using 100 pairs of random primers, 12 bands showed a reproducible induction pattern, and partial sequences of 8 genes were cloned.

蛞蝓进行气味-味觉关联学习，目前的工作旨在利用生理学和分子生物学方法阐明气味学习的机制。唇神经的高频刺激是无条件刺激的体外模拟，引起前大脑 (PC) 中局部 Meld 电位振荡的频率增加，以及破裂的 PC 神经元的兴奋和对 PC 神经元的抑制。非爆发PC神经元。由于非爆发神经元被认为是 PC 的主要神经元，并且可能介导从 PC 到其他大脑区域的信息输出，因此这些结果表明来自 PC 的信息输出在调节过程中减少。对触手神经进行高频刺激，导致PC诱发电位增强，持续时间超过2小时。 PC 中突触传递效率的这种可塑性变化可能与气味学习有关。注射蛋白质合成抑制剂茴香霉素或放线菌酮在条件改变 2-7 天后会损害记忆保留，表明蛋白质合成对于长期记忆保留是必要的。 -术语记忆。我们使用 PCR 差异显示方法，在配对和未配对条件动物的大脑中进一步探索了由气味-味道关联条件反射诱导的基因。使用 100 对随机引物，12 个条带显示出可重复的诱导模式，并克隆了 8 个基因的部分序列。