Molecular neurobiology of olfactory learning in the land slug

陆蛞蝓嗅觉学习的分子神经生物学

基本信息

批准号：
15390010
负责人：
KIRINO Yutaka
金额：
$ 9.47万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15390010/
关键词：
Land slug Olfactory aversive learning Procerebral lobe Differential display Activity marker gene Conditioned stimulus Unconditioned stimulus Synaptic plasticity 神経可塑性シナプス電位前脳嗅覚学習遺伝子発現 differential display

项目摘要

We examined whether the PC is necessary for olfactory learning. In the first series of experiments, slugs were aversively conditioned by pairing vegetable odor with quinidine 7 days after ablation of bilateral PCs. The slugs with PC lesions showed significantly lower scores of learning compared to sham-operated slugs. In the second series of experiments, slugs were first conditioned, and at various time points, the PCs were ablated. When tested 3 days after PC lesion, these slugs also showed severe impairment of memory. These results indicate that the PC is necessary at least for the retention or retrieval of aversive odor memories.We then investigated whether the central synapses in the slug display synaptic plasticity. A single electrical stimulation of the tentacle nerve evoked a potential in the procerebrum (PC) with at least 3 components, and this stimulus was used as the test stimulus. After a high frequency stimulation of the tentacle nerve, the positive component of the evoked potential increased, and this potentiation lasted for more than two hours. That synaptic plasticity found in the PC may be involved in olfactory learning. We also screened genes in the brain that are upregulated by neural activity. Dissecting the brain results in transient up-regulation of neural activity, and this induces transcription of several genes. By differential display analyses using 72 pairs of primers, approximately 10,000 bands were displayed and 28 showed reproducible changes in the expression levels, and 10 of these were cloned for further analysis.

我们研究了电脑对于嗅觉学习是否是必要的。在第一个系列的实验中，在双侧 PC 消融后 7 天，通过将蔬菜气味与奎尼丁配对来对蛞蝓进行厌恶性条件反射。与假手术的蛞蝓相比，有 PC 损伤的蛞蝓表现出明显较低的学习分数。在第二组实验中，首先对蛞蝓进行调节，然后在不同的时间点，PC被消融。 PC 损伤三天后进行测试时，这些蛞蝓也表现出严重的记忆障碍。这些结果表明，PC 至少对于保留或检索令人厌恶的气味记忆是必要的。然后我们研究了蛞蝓的中央突触是否表现出突触可塑性。对触手神经的单次电刺激会在前脑（PC）中诱发至少 3 个分量的电位，并将该刺激用作测试刺激。对触手神经进行高频刺激后，诱发电位的正成分增加，这种增强持续了两个多小时。电脑中发现的突触可塑性可能与嗅觉学习有关。我们还筛选了大脑中受神经活动上调的基因。解剖大脑会导致神经活动短暂上调，从而诱导多个基因的转录。通过使用 72 对引物的差异显示分析，显示了大约 10,000 个条带，其中 28 个条带显示出可重复的表达水平变化，其中 10 个被克隆用于进一步分析。

项目成果

期刊论文数量（106）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Distribution of serotonin-like inununoreactive neurons in the slug Limax valentianus

瓦伦氏蜗牛体内血清素样无免疫反应神经元的分布

DOI：
发表时间：
2004
期刊：
Cell Tiss.Res. 315
影响因子：
0
作者：
Chaya;N.;Terauchi;K.;Yamagata;Y.;Kinjo;J.;Okabe;H.;Mamoru Fukuchi;M.Shimada;Takaaki Shirahata et al.
通讯作者：
Takaaki Shirahata et al.

Ito, I., Watanabe, S., Kimura, T., Kirino, Y., Ito, E.: "Distributions of GABA immunoreactive and acetylcholinesterase-contanig cells in the primary olfactory system in the terrestrial slug Limax marginatus."Zool.Sci.. 20. 1337-1346 (2003)

Ito, I.、Watanabe, S.、Kimura, T.、Kirino, Y.、Ito, E.：“陆生蛞蝓 Limax marginatus 初级嗅觉系统中 GABA 免疫反应性和乙酰胆碱酯酶细胞的分布。”Zool。