Molecular neurobiology of olfactory learning in the land slug

陆蛞蝓嗅觉学习的分子神经生物学

基本信息

批准号：
15390010
负责人：
KIRINO Yutaka
金额：
$ 9.47万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15390010/
关键词：
Land slug Olfactory aversive learning Procerebral lobe Differential display Activity marker gene Conditioned stimulus Unconditioned stimulus Synaptic plasticity 神経可塑性シナプス電位前脳嗅覚学習遺伝子発現 differential display

项目摘要

We examined whether the PC is necessary for olfactory learning. In the first series of experiments, slugs were aversively conditioned by pairing vegetable odor with quinidine 7 days after ablation of bilateral PCs. The slugs with PC lesions showed significantly lower scores of learning compared to sham-operated slugs. In the second series of experiments, slugs were first conditioned, and at various time points, the PCs were ablated. When tested 3 days after PC lesion, these slugs also showed severe impairment of memory. These results indicate that the PC is necessary at least for the retention or retrieval of aversive odor memories.We then investigated whether the central synapses in the slug display synaptic plasticity. A single electrical stimulation of the tentacle nerve evoked a potential in the procerebrum (PC) with at least 3 components, and this stimulus was used as the test stimulus. After a high frequency stimulation of the tentacle nerve, the positive component of the evoked potential increased, and this potentiation lasted for more than two hours. That synaptic plasticity found in the PC may be involved in olfactory learning. We also screened genes in the brain that are upregulated by neural activity. Dissecting the brain results in transient up-regulation of neural activity, and this induces transcription of several genes. By differential display analyses using 72 pairs of primers, approximately 10,000 bands were displayed and 28 showed reproducible changes in the expression levels, and 10 of these were cloned for further analysis.

我们检查了PC是否是嗅觉学习所需的。在第一系列实验中，双边PC消融后7天，通过将蔬菜气味与奎尼丁配对的抗态度。与假手术的sl相比，带有PC病变的SLUGS的学习得分明显较低。在第二系列实验中，首先要调节sl，在各个时间点，PC被消融。当PC病变后3天进行测试时，这些sl骨也显示出严重的记忆力损害。这些结果表明，PC至少对于保留或检索厌恶气味记忆是必需的。然后，我们研究了SLUG中的中央突触是否显示出突触可塑性。触手神经的单个电刺激引起了Procerebrum（PC）的潜力，至少有3个成分，并且该刺激被用作测试刺激。高频刺激了触手神经后，诱发电势的正成分增加了，这种增强持续了两个多小时。在PC中发现的突触可塑性可能与嗅觉学习有关。我们还筛选了由神经活动上调的大脑中的基因。解剖大脑会导致神经活动的短暂上调，这会诱导几种基因的转录。通过使用72对引物的差分显示分析，显示了大约10,000个频段，并显示了28对表达水平可重现的变化，其中10个被克隆了以进行进一步分析。

项目成果

期刊论文数量（106）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Ito, I., Watanabe, S., Kimura, T., Kirino, Y., Ito, E.: "Distributions of GABA immunoreactive and acetylcholinesterase-contanig cells in the primary olfactory system in the terrestrial slug Limax marginatus."Zool.Sci.. 20. 1337-1346 (2003)

Ito, I.、Watanabe, S.、Kimura, T.、Kirino, Y.、Ito, E.：“陆生蛞蝓 Limax marginatus 初级嗅觉系统中 GABA 免疫反应性和乙酰胆碱酯酶细胞的分布。”Zool。

DOI：
发表时间：
期刊：
影响因子：
0
作者：
通讯作者：

Odor-evoked responses in the olfactory center neurons in the terrestrial slug.

DOI：
10.1002/neu.10295
发表时间：
2004-02
期刊：
Journal of neurobiology
影响因子：
0
作者：
M. Murakami;Satoshi Watanabe;Tsuyoshi Inoue;Y. Kirino
通讯作者：
M. Murakami;Satoshi Watanabe;Tsuyoshi Inoue;Y. Kirino

Distribution of serotonin-like inununoreactive neurons in the slug Limax valentianus

瓦伦氏蜗牛体内血清素样无免疫反应神经元的分布

DOI：
发表时间：
2004
期刊：
Cell Tiss.Res. 315
影响因子：
0
作者：
Chaya;N.;Terauchi;K.;Yamagata;Y.;Kinjo;J.;Okabe;H.;Mamoru Fukuchi;M.Shimada;Takaaki Shirahata et al.
通讯作者：
Takaaki Shirahata et al.

Interaction of N-terminal acetyltransferase with the cytoplasmic domain of β-amyloid precursor protein and its effect on Aβ secretion