Development of micro-biosensors

微生物传感器的开发

• 批准号: 03044047
• 负责人: KARUBE Isao
• 金额: $ 3.52万
• 依托单位: University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for international Scientific Research
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03044047/
• 关键词: Biosensor; Environmental Analyses; Micro-biosensor; Enzyme sensor; Clinical testing; immobilized enzyme

Biosensor is a novel analytical system which is available for clinical testing, industrial process control and environmental monitoring. Recently Prof. Karube's group has succeeded miniaturization and mass production of biosensors using silicon fabrication technology. This project aims at promotion of cooperational researches with French and Germany groups. We especially focus on development of micro-biosensors and their applications.Prof. Tamiya stayed at Braunschweig Institute Technology and investigated odorant sensor using piezoelectric crystal coated with artificial lipid which newly was synthesized by prof. Schmidt group. Dr. Tamiya examined possiblity of stable odorant sensor. He discussed with prof. Marty. Perpignan Univ. about pesticide sensor. Prof. Marty's doctor student will stay for 1 and half year at Prof. Karube's lab to promote this project.Dr. Yokoyama stayed at Prof. Comtat lab in Paul Sabatier University. Prof. Comtat has studied electrochemical NAD recyclng system a … More nd applied to biosensors using dehydrogenases. Dr. Yokoyama fabricated microbiosensors for determination of lactic acid using a miro Pt electrode immobilized lactate dehydrogenased and diaphorase. This ia one of actual products supported by this cooperational project.Dr. Suzuki investigated researches about immobilization of enzymes for integration of micro-biosensors. Photocrosslinking polymer used in prof. Karube lab is useful for immobilization of enzymes on multi-printed electrodes which are produced by Prof. Schmidt lab in Braunschweig Institute Technology. Prof. Coulet in Lyon Univ. used a simple immobilization method. Only immersion in enzyme solution gives covalent bonding between membrane and enzyme. Prof. Kubo discussed with Prof. Thomas in Compiegne Univ. who was interested in sensors for control of bioreactors. Minituarized and integrated bosensors are powerful tools for monitoring of bioreactors. Prof. Kubo visited Prof. Marty lab in Perpignan Univ. and discussed about biosensors for environmental analyses. Carbon dioxide sensor is requried for fabrication of French pecicide sensors. Japanese group supply carbon dioxide sensors to French side in order to support French group.Dr. Seki Madea colloboration with Prof. Coulet in Lyon University. He fabricated ISFET biosensor based on LB membrane. ISFET is one of useful transducers for micro-biosensors. LB techniques may be powerful tool for immobilization of biomaterials.Prof. Sato and Kajiwara also took parts in this project. They visited Compiegne Univ. Paul Sabatie Univ. and Perpignan Univ. in order to investigate researches about novel biosensors based on thermal detection.Prof. Karube discussed with Prof. Thomas and Prof. Schmidt about promotion of this project. They concluded that this project should be continued for making fruitful results. Less

生物传感器是一种新型的分析系统，可用于临床测试，工业过程控制和环境监测。最近，Karube教授的小组使用硅制造技术成功地将生物传感器的小型化和批量生产生产。该项目旨在促进与法国和德国团体的合作研究。我们尤其专注于微生物传感器及其应用的开发。 Tamiya住在Braunschweig Institute技术，并使用涂有人工脂质的压电晶体研究了Odorint传感器，该晶体是由教授新合成的。施密特集团。塔米亚（Tamiya）博士检查了稳定的气形传感器。他与教授讨论。马蒂。 Perpignan大学。关于农药传感器。马蒂教授的医生学生将在Karube教授的实验室住1年半，以推广该项目。 Yokoyama留在Paul Sabatier大学的Comtat Lab教授。 COMTAT教授具有使用脱氢酶的生物传感器进行的电化学NAD回收系统。横山博士使用固定的刀片脱氢酶和diaphorase的miro pt电极来确定乳酸，以确定乳酸。这是该合作项目支持的实际产品之一。铃木研究了有关将酶固定在微生物传感器整合的研究。 Prof中使用的PhotocropoSlinking聚合物。 Karube Lab可用于将酶固定在Braunschweig Institute技术中的Schmidt Lab教授生产的多纹理电极上。里昂大学的库尔特教授。使用了一种简单的固定方法。只有浸入酶溶液中才能在膜和酶之间进行共价键合。 Kubo教授与Compiegne大学的Thomas教授讨论了。他对控制生物反应器的传感器感兴趣。摄影和集成的身体是监测生物反应器的强大工具。 Kubo教授在Perpignan大学访问了Marty Lab教授。并讨论了用于环境分析的生物传感器。二氧化碳传感器被重新制造以制造法国专业传感器。日本集团向法国方面提供二氧化碳传感器，以支持法国组。 Seki Madea与里昂大学的Coulet教授融化。他根据LB膜制造了ISFET生物传感器。 ISFET是微生物传感器的有用传感器之一。 LB技术可能是固定生物材料的强大工具。 Sato和Kajiwara也参与了该项目。他们访问了Compiegne大学。保罗·萨巴蒂大学。和Perpignan大学。为了研究基于热检测的新生物传感器的研究。 Karube与托马斯教授和施密特教授讨论了该项目的促进。他们得出的结论是，应该继续进行该项目，以取得富有成效的结果。较少的

项目成果

I. Karube: NTT. Pub.Baioerekutoronikusu no Mirai (in Japanese), 226 (1991)

I. 卡鲁部：NTT。

S.Lee,M.Suzuki,E.Tamiya,I.Karube: "Microbial detection of toxic compounds utilizing recombinant DNA technology and biolaiuinesceuce" Anal.Chim.Acta. 244. 201-206 (1991)

S.Lee、M.Suzuki、E.Tamiya、I.Karube：“利用重组 DNA 技术和生物莱因塞斯对有毒化合物进行微生物检测”Anal.Chim.Acta。

S. M. Chang, Y. Iwasaki, M. Suzuki, E. Tamiya, I. Karube: "Detection of odorants using an array of piezoelectric crystals and neural-network pattern recognition" Anal. Chim. Acta. 249. 323-329 (1991)

S. M. Chang、Y. Iwasaki、M. Suzuki、E. Tamiya、I. Karube：“使用压电晶体阵列和神经网络模式识别检测气味”。

Izumi Kubo,Masako Inagawa,Takako Sugawara,Yoshiko Arikawa,Isao Karube: "Phosphate sensor composed from immobilized pyruvate oxidase and an oxygen electrode" Anal.Letters. 24. 1711-1727 (1991)

Izumi Kubo、Masako Inakawa、Takako Sukawara、Yoshiko Arikawa、Isao Karube：“由固定化丙酮酸氧化酶和氧电极组成的磷酸盐传感器”Anal.Letters。