Development of a monitoring system of biochemical tumor markers during surgery operation

手术过程中生化肿瘤标志物监测系统的研制

基本信息

批准号：
17300176
负责人：
KARUBE Isao
金额：
$ 8.26万
依托单位：
Tokyo University of Technology
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17300176/
关键词：
biosensor tumor marker biochemical compound surgery operation cerebral tumor SPR glucose cancer

项目摘要

In this research we have developed a detection system for biochemical tumor markers with a probe type biosensor based on surface plasmon resonance(SPR). We expect that the biosensor can be applied to a rapid detection of tumors during the surgery by inserting the probe around the affected area.In this research period, we have developed a probe type biosensor and attempted to measure C-reactive protein(CRP), as an example of the tumor markers. It was found that the biosensor can detect 5 □g/m1 of CRP. Using this probe, we have also developed a glucose sensing probe because glucose exists at high concentration around tumor. For this, an enzyme-chromogenic method was applied to detect hydrogen peroxide, which is intermediate of glucose sensing reaction. As the results, we could successfully develop the enzyme-chromogenic SPR biosensor probe for hydrogen peroxide detection.In vitro selection of DNA aptamers which bind to8-Hydroxy-2'-deoxyguanosine(8-OHdG), one of oxidative stress markers around tumor, was demonstrated. Sequence analysis of sequenced 24 clones of the DNA aptamers revealed that all clones contained a conserved sequence with 26 nucleotides length. A DNA probe with the conserved sequence could be applied to the evaluation of oxidative stress of tissues around the tumor in combination with SPR sensor probes.At last, we developed new markers to match the MRI image and the position of the sensor probe was attempted. The markers, named Hybrid marker , had two functions ; one was a contrast agent for MRI, the other was a reflective ball for position measurement. A phantom test showed that the hybrid markers should place inside the area of 50mm far from the receiver coil. An analysis for the past clinical cases by conventional marker exhibited that an angle of the plane contained 80% cases from 40 to 69 degrees.

在这项研究中，我们开发了一种基于表面等离子体共振（SPR）的探针型生物传感器的生化肿瘤标记的检测系统。我们希望通过插入受影响区域的探针在手术期间快速检测生物传感器。在本研究期间，我们开发了一种探针类型的生物传感器，并试图测量C反应蛋白（CRP），作为肿瘤标记的一个例子。发现生物传感器可以检测5□g/m1的CRP。使用此探针，我们还开发了一种葡萄糖感应探针，因为葡萄糖在肿瘤周围高浓度时存在。为此，应用酶 - 染色性方法检测过氧化氢，该方法是葡萄糖传感反应的中间体。作为结果，我们可以成功地开发酶 - 染色的SPR生物传感器探针进行过氧化氢检测。在体外选择DNA适体与8-羟基-2'-脱氧鸟苷（8-OHDG）结合的DNA适体，这是肿瘤周围的氧化应激标志物（8-OHDG）。测序的24个DNA适体克隆的序列分析表明，所有克隆都包含具有26个核苷酸长度的保守序列。具有保守序列的DNA探针可以应用于肿瘤周围Timuli的氧化应激与SPR传感器问题的评估。最后，我们开发了新的标记以匹配MRI图像和传感器探针的位置。标记，名为Hybrid Marker，具有两个功能。一个是MRI的对比剂，另一个是用于位置测量的反射球。幻影测试表明，混合标记应放置在远离接收器线圈的50mm区域内。对过去临床病例的分析传统标记物表明，平面的角度含有40％至69度的病例。

项目成果

期刊论文数量（0）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Awake surgery and functional mapping .

清醒手术和功能标测。

DOI：
发表时间：
2005
期刊：
Nihon Rinsyo 63(9)
影响因子：
0
作者：
Y.Muragaki;T.Maruyama;Y.Iseki;H.Takakura;M. Hori
通讯作者：
M. Hori

Usefulness of intraoperative magnetic resonance imaging for glioma surgery Acta Neurochir [Suppl]

术中磁共振成像在神经胶质瘤手术中的作用 Acta Neurochir [增刊]

DOI：
发表时间：
2006
期刊：
Acta Neurochir [Suppl] 98
影响因子：
0
作者：
Y.Muragai;H.Iseki;T.Maruyama;T.Kawamata;F. Yamane;R.Nakamura;O.Kubo;K.Takakura;T.Hori
通讯作者：
T.Hori

MRI誘導下手術ナビゲーションの自動レジストレーション用ハイブリッドマーカシステムの開発(第1報要求仕様の検討)

开发用于 MRI 引导手术导航自动配准的混合标记系统（第 1 部分：所需规格的检查）