Development of Piezoelectric Immunosensor for the determination of Pyrogens

开发用于测定热原的压电免疫传感器

• 批准号: 63850169
• 负责人: KARUBE Isao
• 金额: $ 4.22万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1989
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-63850169/
• 关键词: Piezo resonator; Viscosity measurement; Pyrogen; Endotoxin; Limulus amebocyte lysate; Gelation reaction; Biosensor; Immunosensor; パイロジエン測定; 水晶振動子; 免疫センサー; インピーダンス測定

The present study aims at the development of rapid and sensitive pyrogen assay system, using piezoelectric quartz crystal for the detection of gelation reaction between Limulus amebocyte lysate and endotoxin, one of the Pyrogens.A piezoelectric quartz crystal is used to monitor viscosity changes in a liquid. A resistance, included in the electrical equivalent circuit of the crystal, and the resonance frequency changes of the crystal, measured by an impedance analyzer, are used for the viscosity measurement. Endotoxin concentrations are determined with this system by the gelation of Limulus amebocyte lysate. The gelation time is computed by using an approximation to a polynomial equation from the resistance or the resonance frequency change. the gelation time measured was in good agreement with that obtained conventionally. The detection limit is 1 pg.ml-l. The maximum rate of change of the resistance was confirmed as a good index of endotoxin concentration in order to shorten the measurement time to <less than or equal> 40 min. The changes in the resistance or resonance frequency were also considered as indices of endotoxin concentrBased on these results, the 6-channel gelation detector using piezoelectric quartz crystals was manufactured by way of trial. With this apparatus, the similar results were obtained for the determination of endotoxin.

本研究的目的是利用压电电石英晶体的快速和敏感的热原测定系统的开发，以检测利用叶酸酯和内毒素之间的凝胶化反应。通过阻抗分析仪测量的晶体的电气等效电路中包含的电阻，用于粘度测量。内毒素浓度通过该系统确定，通过limulus amebocyte裂解物的凝胶化。通过使用电阻或共振频率变化对多项式方程的近似值来计算凝胶时间。测得的凝胶时间与常规获得的时间非常吻合。检测极限为1 pg.ml-l。电阻的最大变化速率被确认为内毒素浓度的良好指数，以便将测量时间缩短到<小于或等于> 40分钟。电阻或共振频率的变化也被视为浓缩这些结果的内毒素的指标，使用压电石英晶体的6通道胶凝检测器是通过试验制造的。使用该设备，获得了类似的结果来确定内毒素。

项目成果

Muramatsu,H.,Tamiya,E.Suzuki,M.,Karube,I.: "A Quarts Crystal Geletion Deterctor for Determination of Fibrinogen Concentration" Analytica Chimica Acta. 217. 321-326 (1989)

Muramatsu,H.,Tamiya,E.Suzuki,M.,Karube,I.：“用于测定纤维蛋白原浓度的石英晶体凝胶检测器”分析化学学报。

I. Karube and E. Tamiya: Frontiers in Bioprocessing. CRC Press, Inc., Florida, 456 (1990)

I. Karube 和 E. Tamiya：生物加工前沿。

Muramatsu,H.,Tamiya,E.Suzuki,M.,Karube,I.: "Viscosity Monitoring with a Piezoelectric Quarts Crystal and Its Application to Determination of Endotoxin by Gelation of Limulus Amebocyte Lysate" Analytica Chimica Acta. 215. 91-98 (1988)

Muramatsu,H.、Tamiya、E.Suzuki,M.、Karube,I.：“用压电石英晶体进行粘度监测及其在鲎变形细胞裂解物凝胶化测定内毒素中的应用”分析化学学报。

Isao Karube(分担執筆): "BIOTEC2:Biosesors and Environmental Biotechnology" Gustav Fischer,Stuttgart, 160 (1988)

Isao Karube（贡献者）：“BIOTEC2：Biosesors 和环境生物技术”Gustav Fischer，斯图加特，160 (1988)

I.Karube: "Biosensor;Yearbook Biotechnology Band 2" Carl Hanser Ver, 183-206 (1988)

I.Karube：“生物传感器；年鉴生物技术带 2”Carl Hanser Ver，183-206 (1988)