Development of Third Generation Biosensors

第三代生物传感器的开发

• 批准号: 07044129
• 负责人: KARUBE Isao
• 金额: $ 7.42万
• 依托单位: University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for international Scientific Research
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07044129/
• 关键词: cyanide; phosphate; artificial diamond; genetic algorism; マルチバイオセンサー; 水質モニタ用センサー; 環境計測

Development of multibiosensor for environmetal monitoringBiosensor for cyanide detectionusing resparatory change of the microbe immobilized on the surface of oxygen electrode. This was focused on the future application of river water monitoring. Evaluation of the sensor developed were performed and the comparison woth conventional method was done. According to the discussion with Prof. Marty's group, who has a highly sofisticated technique on environmental monitoring, the sensor seemed to be very useful.Development of multichannel biosensorsSeveral efforts were tried to have the higher sensitivity of the phosphate sensor using chemiluminescence. Research group of Prof. Coulet has already worked with chemiluminescence using luminol and their advice worked well for our purpose.Development of gaseous materials biosensorsElectrophotochemical approach towards the detection of gaseous materials such as cyanide was concluded as effective for the purpose. The use of artificial diamond electrode for biosensor seemed also effective because of its wide potential window without being affected by water.Development of water quality monitoring sensorsThe use of catalytic antibody for a new biosensor seemed very interesting, and the preparation of bio-functional molecules using computer-aided method such as genetic algorism also seemed effective.

开发用于环境监测的多摩托传感器，用于氰化物检测，从而在氧电极表面上固定的微生物的爆炸性变化。这重点是未来的河水监测。进行了开发的传感器评估，并进行了常规方法的比较。根据与Marty教授的讨论，对环境监测具有高度统一的技术，该传感器似乎非常有用。尝试使用化学发光的多通道生物传感器努力的开发具有更高的磷酸盐传感器灵敏度。 Coulet教授的研究小组已经使用Luminol与化学发光合作，他们的建议对我们的目的效果很好。生物传感器核代理化学方法的开发用于检测气态材料（例如氰化物），以此为目的有效。人造钻石电极在生物传感器中的使用似乎也有效，因为它的潜在窗口很广，而不会受到水的影响。水质监测传感器的开发催化抗体对新生物传感器的使用似乎非常有趣，并且制备了使用诸如遗传algorism的计算机辅助方法的生物官能分子的制备也似乎也有效。

项目成果

Kazunori Ikebukuro,Isao Karube,et al.: "Phosphate sensing system using pyruvate oxydase and chemiluminescence detection" Biosensors and Bioelectronics. (in press).

Kazunori Ikebukuro、Isao Karube 等人：“使用丙酮酸氧化酶和化学发光检测的磷酸盐传感系统”生物传感器和生物电子学。

Kazunori Ikebukuro,Isao Karube et al.: "A novel biosensor system for the determination of phosphate" Journal of Biotechnology. (in press).

Kazunori Ikebukuro、Isao Karube 等人：“一种用于测定磷酸盐的新型生物传感器系统”生物技术杂志。

Kazunori Ikebukuro,Isao Karube et al.: "A novel biosensor system for cyanide based on the chemiluminescence reaction" Analytica Chimica Acta. (in press).

Kazunori Ikebukuro、Isao Karube 等人：“基于化学发光反应的新型氰化物生物传感器系统”Analytica Chimica Acta。

Jeong Im Lee,Isao Karube: "Development of a biosensor for gaseous cyanide in solution" Biosensors & Bioelectronics. 11. 1147-1154 (1996)

Jeong Im Lee、Isao Karube：“溶液中气态氰化物生物传感器的开发”

I.Karube,Y.Nomura,Y.Arikawa: "Biosensor for environmental control" Trends Anal Chem.14. 295-299 (1995)

I.Karube、Y.Nomura、Y.Arikawa：“用于环境控制的生物传感器”趋势 Anal Chem.14。