Exposure to Mixtures of Emerging Contaminants in the Environment - Are Communities in Uganda at Health Risk?- A Case Study of Mbarara City.

接触环境中新兴污染物的混合物 - 乌干达的社区面临健康风险吗？ - 姆巴拉拉市的案例研究。

• 批准号: 10732272
• 负责人: Grace Birungi
• 金额: $ 10.81万
• 依托单位: MBARARA UNIVERSITY/SCIENCE/ TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-07-27 至 2028-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10732272
• 关键词: Adverse effects; Affect; Africa; African; Americas; Animals; Aromatic Polycyclic Hydrocarbons; Arsenic; Asia; Biological; Biological Markers; Biological Process; Cardiovascular Diseases; Case Study; Cessation of life; Chemicals; Chromatography; Chronic; Cities; Communities; Control Groups; Country; Data; Data Analyses; Developed Countries; Development; Development Plans; Diagnosis; Disease; Doctor of Philosophy; Ecosystem; Environment; Environmental Health; Europe; Exposure to; Fluorides; Goals; Grant; Health; Health Priorities; Heavy Metals; Histologic; Histopathology; Human; Human Resources; Industrialization; Intervention; Knowledge; Leadership; Link; Malignant Neoplasms; Mammals; Manuscripts; Mentors; Mentorship; Metabolic; Metabolism; Methods; Monitor; Morbidity - disease rate; Municipalities; Mus; National Institute of Environmental Health Sciences; Nitrates; Organ; Pathway interactions; Persons; Pesticides; Plants; Pollution; Prognosis; Public Health; Publications; Recommendation; Reporting; Research; Research Methodology; Research Personnel; Risk; Risk Assessment; Risk Estimate; Science; Secure; Serum; Source; Statistical Data Interpretation; Structure; Surface; System; Techniques; Technology; Testing; Training; Uganda; Universities; Water; Water Stress; Work; Writing; albino mouse; anthropogenesis; biomarker validation; candidate identification; career; career development; disorder prevention; disorder risk; drinking water; environmental toxicology; ground water; high risk; insight; low and middle-income countries; metabolic profile; metabolome; metabolomics; mortality risk; pollutant; prevent; responsible research conduct; skills; wastewater epidemiology

Project Summary/Abstract: River Rwizi the main source of water for Mbarara City and surroundings is threatened by anthropogenic pollution. Preliminary studies detected pesticides, polycyclic aromatic hydrocarbons and heavy metals at concentrations higher than WHO drinking water recommendations, exposing over 91,964 City residents to pollutants. Pollutant exposure is linked to Non-Communicable Diseases (NCDS) and emerging pollutants (EPs) pose new threats to human health. No study has fully characterized EPs in River Rwizi or wastewater treatment plants (WWTPs) in its catchment yet EPs and wastewater have an inextricable link. It is hypothesized that the changing anthropogenic activities in Mbarara City have increased the concentrations of EPs in River Rwizi, exposing residents to higher risks of NCDs through perturbations of the metabolome. This study will inform intervention strategies to reduce environmentally induced diseases in LMICs contributing to NIEHS goals and Uganda’s health priorities of disease prevention, mitigation and control. Candidate: I have skills in research methodology, data analysis, scientific presentation, and manuscript writing accrued during my BS, MSc and PhD training and research works that resulted in 23 publications so far. This K43 grant will secure me training and mentorship in: advanced research methodology, management of large data, research administration, responsible conduct of research, and grant writing. This proposed career development plan will build on my previous training to facilitate me to become an independent investigator through five training goals: 1) execution of advanced qualitative and quantitative 1H-NMR and LC-MS metabolomics; 2) advanced statistical analyses; 3) interpretation of metabolomics data in the context of biological processes; 4) strengthening leadership and professional skills; and 5) application of histopathology to chronic exposure studies. Mentors and Environment: This plan will benefit from physical and human resources available through Mbarara University of Science and Technology [through Dr. Tusubira and Prof. Alele (Biochemist and Toxicologist)], the Universities of San Diego and Makerere [Profs. Bolender and Ntale, Chemists]. Research: I will (i) Identify and quantify EPs in River Rwizi and in WWTPs in its catchment using chromatographic techniques and Wastewater Epidemiology approach. (ii) The effect of exposure to EPs will be studied by metabolomics and histopathology approaches. My short-term goal is to identify candidate metabolites associated with exposure to EPs while my long term goal is to develop a career in Environmental toxicology. Towards the end of this support I will apply for an R01.

项目摘要/摘要：rwizi河流的主要水源 周围环境受到人为污染的威胁。初步研究检测到农药， 多环芳族烃和重金属的浓度高于饮用者 水建议，将超过91,964个城市居民暴露于污染物中。污染物暴露 与非传染性疾病（NCD）和新兴污染物（EPS）相关联 对人类健康的威胁。没有研究在RWIZI或废水中充分表征EPS 处理厂（WWTPS）在其集水区中，但EPS和废水具有不可分割的联系。它 假设姆巴拉拉市的人为活动不断变化，这增加了 RWizi河中的EPS浓度，通过 代谢组的扰动。这项研究将为干预策略提供信息以减少 LMIC中的环境引起的疾病为NIEHS目标和乌干达的健康做出了贡献 疾病预防，缓解和控制的优先级。候选人：我有研究技能 方法，数据分析，科学演示和手稿写作在我的期间累积 迄今为止，BS，MSC和博士培训和研究工作导致了23份出版物。这个K43 格兰特将确保我的培训和心态：高级研究方法，管理 大数据，研究管理，负责任的研究和授予写作。这 拟议的职业发展计划将基于我以前的培训，以促进我 通过五个培训目标成为独立研究者：1）执行高级 定性和定量1H-NMR和LC-MS代谢组学； 2）高级统计 分析； 3）在生物过程中的代谢组学数据的解释； 4） 增强领导能力和专业技能； 5）将组织病理学应用于慢性 暴露研究。导师和环境：该计划将受益于身体和人类 通过MBARARA科学技术大学获得的资源[通过Dr. Tusubira和Alele教授（生物化学家和毒理学家）]，圣地亚哥大学和 Makerere [教授。 Bolender和Ntale，化学家]。研究：我将（i）识别和量化EPS 河流RWIZI和WWTPS在其集水区使用色谱技术和废水 流行病学方法。 （ii）暴露于EPS的影响将由代谢组学研究和 组织病理学方法。我的短期目标是确定与候选代谢物相关的代谢物 接触EPS，而我的长期目标是发展环境职业 毒理学。在此支持结束时，我将申请R01。