Development of biocompatible metal-BMP composit

生物相容性金属-BMP复合材料的开发

基本信息

批准号：
02670882
负责人：
KAWAI Tatsushi
金额：
$ 1.47万
依托单位：
School of Dentistry Aichigakuin University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1991
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02670882/
关键词：
Implant OSTEOGENESITY Bone morphogenetic protein BMP Biomaterial Titanium Composit material Biocompatible metal

项目摘要

In order to prepare water soluble BMP,S-200 gel filtration was performed, then higher molecular weight than 40,000 Da was cut off. The fraction of 10,000-30,000 Da was dialyzed against water and centrifuge out the ppt. The supernatant was lyophilized. This was water soluble BMP.This BMP and powdered pure metals(Ag,Al,Au,Co,Cr,Cu,Fe,Ge,In,Ir,Mn,Mo,Ni,Pb,Pd,Pt,Sb,Sn,Ta,Ti,W,Zn)were combined, then the effect of each metals on new bone formation was investigated. In the implantation of Au,Mo,Ti, there were no inhibition of new bone formation under the existence of these metals. On the contrary, Cu,Fe,Sb suppressed osteoinduction remarkably and new bone formation did not occur completely. With other metals, new bone formation were observed but the amounts of new bone were decreased. Uptake of [3H]-thyamidine, [35]-sulfate were measured to know the DNA synthesis, GAG (glycosaminoglycan) for the quantitative analysis of cytotoxity and chondrogenesis by Au,Pt,Ni,Ti.Au,Pt.Ti did not suppress the DNA synthesis, and Ni did remarkably. Same phenomena was obtained in GAG synthesis. In some condition Pt inhibit osteoactivity slightyl, however in usual experimental condition, it did not effect on new bone formation. Consequently, Au-Pt alloy seems to be a excellent candidate for the developing biocompatible alloy. Addition of Mo to Au-Pt will be a good modification. Ti showed excellent property for a biocompatible metal mentioned as previously.

为了制备水溶性BMP，进行S-200凝胶过滤，然后截除高于40,000 Da的分子量。 10,000-30,000 Da 的级分用水透析并离心出 ppt。将上清液冻干。这是水溶性BMP。该BMP和粉末状纯金属(Ag,Al,Au,Co,Cr,Cu,Fe,Ge,In,Ir,Mn,Mo,Ni,Pb,Pd,Pt,Sb,Sn,Ta ，Ti，W，Zn）组合，然后研究每种金属对新骨形成的影响。在Au、Mo、Ti的植入中，这些金属的存在对新骨的形成没有抑制作用。相反，Cu、Fe、Sb显着抑制骨诱导，新骨形成不完全发生。对于其他金属，观察到新骨形成，但新骨量减少。测量[3H]-胸苷、[35]-硫酸盐的摄取以了解DNA合成，GAG（糖胺聚糖）用于细胞毒性和软骨形成的定量分析，Au,Pt,Ni,Ti.Au,Pt.Ti不抑制DNA 合成，Ni 的表现非常出色。在GAG合成中也得到了同样的现象。在某些情况下，Pt会轻微抑制骨活性，但在通常的实验条件下，它不会影响新骨的形成。因此，Au-Pt 合金似乎是开发生物相容性合金的绝佳候选者。在 Au-Pt 中添加 Mo 将是一个很好的改性。作为前面提到的生物相容性金属，Ti 显示出优异的性能。