Osteoinduction derived from cartilage, which induced on a composite polymer membrane with BMP

源自软骨的骨诱导，在带有 BMP 的复合聚合物膜上进行诱导

基本信息

批准号：
14571873
负责人：
KAWAI Tatsushi
金额：
$ 2.18万
依托单位：
Aichi-Gakuin University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2003
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571873/
关键词：
BMP Bone morphogenetic protein poly-saccharide Biomaterial Functionalmaterial Implant Bone induce Membrane

项目摘要

Extraction of BMP in large scale, selection of scaffold, cartilage induction on a membrane material and osteoinduction in the host body by re-implant of induced cartilage were experimented. Extraction of BMP is on the line in stable, and at least 1000mg of BMP is obtained per month. Considering BSE problem, starting material of BMP was changed to pig bone from bovine bone. There was no difference between pig and bovine BMP in the ability of bone induction. Selection of polymers showing biological function both in vivo and in vitro was performed. Candidates of polymers were attelo collagen, gelatin, agarose, poly-saccharide, PLA-PLGA co-polymer, polyehylmethacrylate and polytetrafluoroethylene.Almost all polymers showed excellent property as BMP carriers, eventually, polytetrafluoroethylene was selected as a membrane forming material. PLA-PLGA co-polymer, polyehylmethacrylate and methylcellulose will be important material for this research project in future. Porous titanium was also investigated, and showed that osteoprogenitor cells were well harvested in the titanium pore. Cartilage formation was occurred on the polytetrafluoroethylene membrane by inducing factor of BMP. This Cartilage system was implanted into subcutaneous tissue of rat, which cause the osteoinduction at 3 weeks after implantation. The objective of this project was then achieved by this result.

进行了大规模提取BMP、支架的选择、膜材料上的软骨诱导以及通过重新植入诱导软骨在宿主体内进行骨诱导的实验。 BMP提取稳定上线，每月至少获得1000mg BMP。考虑到BSE问题，BMP的起始材料由牛骨改为猪骨。猪和牛BMP的骨诱导能力没有差异。选择在体内和体外均表现出生物功能的聚合物。候选的聚合物有attelo胶原蛋白、明胶、琼脂糖、多糖、PLA-PLGA共聚物、聚甲基丙烯酸乙酯和聚四氟乙烯。几乎所有聚合物都表现出优异的BMP载体性能，最终选择聚四氟乙烯作为成膜材料。 PLA-PLGA共聚物、聚甲基丙烯酸乙酯和甲基纤维素将是未来该研究项目的重要材料。还对多孔钛进行了研究，结果表明在钛孔中可以很好地收获骨祖细胞。通过BMP诱导因子在聚四氟乙烯膜上形成软骨。将该软骨系统植入大鼠皮下组织，植入后3周引起骨诱导。这个结果就实现了该项目的目标。