Development of BMP-Metal composite

BMP-金属复合材料的开发

• 批准号: 09672023
• 负责人: KAWAI Tatsushi
• 金额: $ 1.98万
• 依托单位: Aichi-gakuin university
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09672023/
• 关键词: BMP; Bone morphogenetic protein; metal; biomaterial; functional material; implant; osteoinduction; dental implant

In order to develop the metal-BMP composites, absorb of the BMP to the surface of metals were investigated, then some metals and BMP were combined together to estimate its osteoinductive activity. Recombinant BMP was synthesized by sf-9cells infected by Baculo virus, which was, inserted the BMP gene. However, the osteoinductive activity of the recombinant BMP was 1/10 of BMP which was extracted from natural resource, Other system was also investigated, but all recombinant BMP showed faint osteoinductive activity. On the contrary, the method of extraction of water soluble BMP derived from bovine bone was established steadily and stable amount of BMP was being extracted. An unknown protein was extracted during the purification of BMP and it was determined as osteonectin by the amino acid analyses. As other proteins contained in bone may act natural carrier of BMP, the purification of that protein is on the experiment. Pure titanium showed excellent affinity to BMP as preliminary experiment. Anodic oxdized treatment was performed on the surface of pure titanium and the specimen with gelatin carrier was estimated remarkably high osteoinductive activity. Sand blasted experimental group showed also significantly high osteogenesity, Generation of highly purified BMP or recombinant BMP and the surface treatment of metals will be main subject of BMP for the clinical investigation.

为了开发金属-BMP复合材料，研究了BMP对金属表面的吸附，然后将一些金属和BMP结合在一起以评估其骨诱导活性。杆状病毒感染sf-9细胞合成重组BMP，并插入BMP基因。然而，重组BMP的骨诱导活性是从天然资源中提取的BMP的1/10，也研究了其他系统，但所有重组BMP都表现出微弱的骨诱导活性。相反，从牛骨中提取水溶性BMP的方法已逐步建立，并且BMP的提取量也稳定。 BMP 纯化过程中提取了一种未知蛋白质，经氨基酸分析确定其为骨连接素。由于骨中含有的其他蛋白质可能是BMP的天然载体，因此该蛋白质的纯化正在进行实验。初步实验显示纯钛对 BMP 表现出优异的亲和力。对纯钛表面进行阳极氧化处理，带有明胶载体的样品估计具有非常高的骨诱导活性。喷砂实验组也表现出显着的高成骨性，高纯度BMP或重组BMP的生成以及金属的表面处理将是BMP临床研究的主要课题。

项目成果

T.Kawai: "New Bone Formation by BMP-Ceramics Composites"Proceedings of 3rd IWCBS. 9(DW0423(CD-ROM)). (1997)

T.Kawai：“BMP-陶瓷复合材料形成新骨”第三届 IWCBS 会议记录。

T.Kawakami,T.Kawai: "Evaluation of Heterotopic Bone Formation Induced by Squalane and Bone Morphogenetic Protein Composite." Clinical Orthopaedics and Related research. 4. 261-266 (1997)

T.Kawakami，T.Kawai：“角鲨烷和骨形态发生蛋白复合物诱导的异位骨形成的评估”。

Tatshushi Kawai, etc.: "Ketaikeisei Bunsimekanizumukennkyuu no saishin gijyutu"Koukuu Hoken Kyoukai. 178-180 (1998)

河合达志等：《Ketaikeisei Bunsimekanizumukennkyuu no saishin gijyutu》Koukuu Hoken Kyoukai。

Kawai,T., Miyazawa,K.: "Quantitative analysis of new bone induced by BMP" Proceedings of 4th IWCBS. PA0074(CD-ROM) (1998)

Kawai,T., Miyazawa,K.：“BMP 诱导的新骨的定量分析”第四届 IWCBS 会议记录。

河合達志: "形態形成・分子メカニズム研究の最新技術"財団法人 口腔保健協会 東京. 2. 178-180 (1998)

Tatsushi Kawai：“形态发生和分子机制研究的最新技术”东京口腔健康协会 2. 178-180 (1998)。