Studies on the Requirement and Nutritional Role of Marine Fish Larvae for EPA and DHA

海水鱼幼体对EPA和DHA的需要量及营养作用的研究

基本信息

批准号：
02660208
负责人：
TAKEUCHI Toshio
金额：
$ 1.41万
依托单位：
Tokyo University of Fisheries
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1991
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02660208/
关键词：
EPA DHA Requirement Nutrition Marine Fish Larvae Artemia マダイシマアジワムシ

项目摘要

This study was investigated the requirement and nutritional role of marine fish larvae for icosapentaenoic acid (EPA) and docosahexaenoic acid (DHA).At first, different stages of larval fish such as striped jack, yellowtail, cod were collected and analysed their fatty acid compositions. Only DHA content in three species was decreased immediately after hatching. This result suggests that fish larvae during 5-10mm size (total length) may require high amount of DHA.Secondly, the experiment was conducted to find out a suitable lipid type for enrichment by comparing the incorporation of lipid and n-3highly unsaturated fatty acids (n-3HUFA) in Artemia nauplii between various types of oil, such as ethylesters (EE), triglycerides (TG), methyl esters (ME), and free fatty acids (FFA) containing about 43% of n-3HUFA, using the so-called direct method. It is concluded that a maximum incorporation of n-3HUFA in Artemia can be obtained by enrichment with EE for 24h and that EE or ME are very quickly … More incorporated into TG fractions. Then these Artemia were fed on larval striped knife jaw and red sea bream to compare the dietary value of Artemia enriched with various types of oil, based on the growth, survival and chemical compositions of both fish larvae. The result indicated that the dietary value of Artemia to both species was effectively improved by enrichmewith TG, ME, and EE, especially with EE.In next, dietary value of Artemia enriched with EE type of EPA and DHA for larval yellowtail and red sea bream was investigated. Both larval yellowtail and red sea bream fed on nauplii enriched with EPA showed good growth and high survival rate, however quite low activity tests were observed. On the other hand, fish fed DHA enriched Artemia showed high feed performances, incl. high activity tests. Thus, it is clearly indicated that larval fish require DHA in order to receive good growth and health.Finally, two feeding experiments were conducted to compare the essential fatty acids (EFA) value between EPA and DHA in juvenile red sea bream and striped jack. Both fish fed a diet without supplemental EFA showed low feed efficiency and high morthlity. The requirement of EPA and DHA was estimated to be around 1% and 0.5% in diet for juvenile red sea bream, respectively. The EFA efficiency of DHA was also found to be twice as high as that of EPA. On the other hand, the experiment on the EFA of juvenile striped jack have demonstrated that : 1) DHA is more effective than EPA as EFA ; 2) requirement for DHA alone is about 1.7% in diet.This study was clearly demonstrated that marine fish larvae require DHA as EFA and physiological purpose. Less

研究了这项研究的海洋鱼幼虫对二糖烯酸（EPA）和Docosahecahexaenoic酸（DHA）的需求和营养作用。首先，收集了幼虫鱼的不同阶段，例如条纹夹克，Yellowtail，cod，COD，并分析其脂肪酸的脂肪酸成分。孵化后，只有三种物种的DHA含量得到了改善。该结果表明，在5-10mm大小（总长度）期间的鱼幼虫可能需要大量的DHA。其次，进行了实验，以比较脂质和N-3高的不饱和脂肪酸（N-3HUFA）在Artemia nauplii中的脂质和N-3高度不饱和脂肪酸（N-3HUFA）之间的富集，以富集的各种油中的各种油中的油脂，例如Ethylesters（EE），触发剂（TG），甲基酯（ME），甲基酸（ME），（me），（me），（me），（me），（me），（me），（me）（me）（me），（me）（ffa） N-3HUFA，使用所谓的直接方法。得出的结论是，最大纳入了TG分数。然后，根据两种鱼类幼虫的生长，生存和化学成分，将这些青蒿脱落在幼虫条纹刀和红海开花中，以比较富含各种油的artemia的饮食价值。结果表明，富集，ME和EE有效地改善了甲art毛的饮食价值，尤其是EE。接下来，研究了富含EE EPA和DHA的Artemia的饮食价值，用于幼虫黄色的幼虫黄色尾巴和红海开花。在富含EPA的Nauplii上喂养的幼虫黄尾巴和红海开花均显示出良好的生长和较高的存活率，但是观察到了相当低的活性测试。另一方面，富含DHA的FISH富含Artemia的鱼表现出很高的饲料表演，包括。高活动测试。这清楚地表明，幼虫鱼需要DHA才能获得良好的生长和健康。在本文中，进行了两个喂养实验，以比较少年红海和条纹千斤顶中EPA和DHA之间的必需脂肪酸（EFA）值。两种没有补充EFA的饮食喂养的鱼都表现出低饲料效率和高敏感性。 EPA和DHA的需求估计分别为少年红海开花的饮食约为1％和0.5％。还发现DHA的EFA效率是EPA的两倍。另一方面，对少年条纹插孔的EFA的实验证明：1）DHA比EPA更有效； 2）仅DHA的要求约为1.7％。这项研究清楚地表明，海洋鱼幼虫需要DHA作为EFA和身体目的。较少的