RETROCONVERSION OF DHA IN LIVE FOOD

活食中 DHA 的逆向转化

• 批准号: 04806029
• 负责人: TAKEUCHI Toshio
• 金额: $ 1.22万
• 依托单位: TOKYO UNIVERSITY OF FISHERIES
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04806029/
• 关键词: ARTEMIA; DHA; RETROCONVERSION; EPA; RADIOACTIVITY; FATTY ACID

The study was investigated whether docosahexaenoic acid (DHA) can be retroconverted to docosapentaenoic acid (DPA) or eicosapentaenoic aicd (EPA) in live food, especially Artemia nauplii. In the first year, Artemia was enriched with DHA in dilrect method. When EPA was supplemented to Artemia medium, the content of EPA in Artemia increased, but DPA and DHA were not detected. In DHA-enriched Artemia, the DHA content was increased, and DPA was detected a little. Furthermore, the EPA content was increased more than that in Artemia enriched with oleic acid (containing no n-3 fatty acids). On the other hand, the DHA content in DHA-enriched Artemia accumulated less than half of the EPA content in EPA content in EPA-enriched Artemia. This result indicated that DHA might be converted to DPA and EPA in Artemia. In order to prove the conversion from DHA to DPA, which is the first step of retroconversion, [1-^<14>C] DHA was enriched directly to Artemia. The result showed that the radioactivity uptake was slightly observed in the DPA fraction by using HPLC technique. In the second year, it was also indicated that the cultural temperature may change the proportion of DHA retroconversion. DHA uptake in Artemia was examined in the experimental range maintained at 16 and 25ﾟC and the range maintained at 16ﾟC for 6h and changed to 25ﾟC As a result, a large difference in DHA uptake was not observed in the isothermal temperature, but in the range with the temperature change radioactive uptake was increased by 30%. This shows that temperature affect on the accumulation of fatty acids in Artemia. It is suggested that Artemia has retrocovert pathway from DHA to EPA, and Artemia use DHA as energy sources, so DHA is hard to accumulate in Artemia.

该研究调查了活体食品中的二十二碳六烯酸（DHA）是否可以逆向转化为二十二碳五烯酸（DPA）或二十碳五烯酸（EPA）。在第一年，卤虫以直接方法富含DHA。添加卤虫培养基后，卤虫中EPA含量增加，但未检测到DPA和DHA。富含DHA的卤虫，DHA含量增加，并且检测到少量DPA，此外，与富含油酸（不含n-3脂肪酸）的卤虫相比，EPA含量增加更多。富含DHA的丰年虫中的DHA含量积累不到富含EPA的丰年虫中的EPA含量的一半。这一结果表明，丰年虫中的DHA可能转化为DPA和EPA。为了证明从DHA到DPA的转化，这是逆向转化的第一步，将[1-^ 14 C]DHA直接富集到卤虫中。结果表明，通过使用，在DPA级分中观察到轻微的放射性吸收。第二年，在维持在16℃和25℃的实验范围内检测培养温度可能会改变卤虫中DHA的摄取比例。 16°C保持6小时，然后改为25°C。结果，在等温温度下，没有观察到DHA摄取的较大差异，但在温度变化的范围内，放射性摄取增加了30%。温度对丰年虫体内脂肪酸积累的影​​响表明，丰年虫具有从DHA到EPA的逆向途径，并且丰年虫以DHA为能源，因此DHA很难在体内积累。卤虫。