Molecular biology mark study on development of digestive and absorptive function of mothr's milk fed rat pups intestine : Developmental changes in protein -Effect of neonatal undernutrition on the subsquent development of protein digestive and absorptive

母乳喂养幼鼠肠道消化吸收功能发育的分子生物学标志研究：蛋白质发育变化-新生儿营养不良对蛋白质消化吸收功能后续发育的影响

基本信息

批准号：
05670975
负责人：
TAKEUCHI Toshio
金额：
$ 1.47万
依托单位：
Showa University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1995
项目状态：
已结题

项目摘要

We have examined the effect of neonatal undernutrition on protein degestive and absorptive function of rat intestine. Eight pups were reared at a litter in the control group, while 16 pups in the undernourished group were reared for 1 week commencing at the age of 2, and then 8 pups were reared as a litter. Protein digestive function of intestinal lumen (proteolytic activity), the protein absorptive function (pinocytic activity) by using everted small intestinal sac, and the Cathepsin B activity of intestinal mucosa and liver were measured at 2,9,16 and 23 days in control and undernourished pups.The proteolytic activity was 7.6,177.3,530.6 (mu g/tripsin equivalent/ml) in the control group, respectively. However, the value of undernourished group showed decreases compared with those in the control group (70.3,46.8 and 98.5% of each opposite control). The mucosal uptake amount of RI labeled BSA at 9 and 16 days were increased significantly in the undernourished group (5.6,1.9 times of control), but the amount of that at 23 days was not significantly defferent from control. On the other hand, that of RI labeled BLG was more increased compared with each opposite control (69.6,2.3,3.4 times). The Cathepsin B activity (U/mg protein/ml) of the control liver at 9,16,23 days showed 10.4,18.1,9.7. The activity of the undernourished group was significantly declined on these days (7.9,10.0,7.1). While, that of intestinal mucosa groups showed significant decreases compared with those in control group at 9 and 16 days (4.4,4.3), but significant increases (2 times the control value) at 23 days.These studies suggest that neonatal undernutrition may have unfavorable influences on the subsequent development of protein digestive and absorptive function, and may be a factor in development of future allergic disease.

我们研究了新生儿营养不良对大鼠肠道蛋白质消化和吸收功能的影响。对照组每窝饲养8只幼崽，营养不良组从2岁开始饲养16只幼崽，饲养1周，然后每窝饲养8只幼崽。分别于第2、9、16、23天测定对照组和对照组的肠腔蛋白质消化功能（蛋白水解活性）、外翻小肠囊的蛋白质吸收功能（胞饮活性）以及肠粘膜和肝脏的组织蛋白酶B活性。营养不良的幼崽。对照中的蛋白水解活性为 7.6,177.3,530.6（μ g/tripsin 当量/ml）组，分别。然而，与对照组相比，营养不良组的数值有所下降（相对对照组分别为70.3%、46.8%和98.5%）。营养不良组第9天和第16天时RI标记BSA的粘膜摄取量显着增加（对照的5.6倍、1.9倍），但第23天时与对照没有显着差异。另一方面，RI标记的BLG与每个相对对照相比增加更多(69.6、2.3、3.4倍)。对照肝脏在第9、16、23天的组织蛋白酶B活性（U/mg蛋白质/ml）显示为10.4、18.1、9.7。营养不良组的活动在这些天显着下降（7.9、10.0、7.1）。而第9天和第16天（4.4、4.3）时，肠粘膜组与对照组相比显着下降，但第23天时显着增加（对照值的2倍）。这些研究表明，新生儿营养不良可能对新生儿的营养不良有不利影响。影响蛋白质消化和吸收功能的后续发展，并可能是未来过敏性疾病发展的一个因素。