Molecular biology mark study on development of digestive and absorptive function of mothr's milk fed rat pups intestine : Developmental changes in protein -Effect of neonatal undernutrition on the subsquent development of protein digestive and absorptive

母乳喂养幼鼠肠道消化吸收功能发育的分子生物学标志研究：蛋白质发育变化-新生儿营养不良对蛋白质消化吸收功能后续发育的影响

• 批准号: 05670975
• 负责人: TAKEUCHI Toshio
• 金额: $ 1.47万
• 依托单位: Showa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05670975/
• 关键词: protein; digestion; absorption; pionocytosis; proteolytic enzyme activity; cathepsin B activity; タンパクの消化吸収; 低栄養; Proteolytic activity; pinocytosis; CathepsinB activity; 母乳栄養; 蛋白消化吸収能; 刷子縁膜酵素活性; Cathepsin B; protein; digestion; absorption; pionocytosis; proteolytic enzyme activity; cathepsin B activity; タンパクの消化吸収; 低栄養; Proteolytic activity; pinocytosis; CathepsinB activity; 母乳栄養; 蛋白消化吸収能; 刷子縁膜酵素活性; Cathepsin B

We have examined the effect of neonatal undernutrition on protein degestive and absorptive function of rat intestine. Eight pups were reared at a litter in the control group, while 16 pups in the undernourished group were reared for 1 week commencing at the age of 2, and then 8 pups were reared as a litter. Protein digestive function of intestinal lumen (proteolytic activity), the protein absorptive function (pinocytic activity) by using everted small intestinal sac, and the Cathepsin B activity of intestinal mucosa and liver were measured at 2,9,16 and 23 days in control and undernourished pups.The proteolytic activity was 7.6,177.3,530.6 (mu g/tripsin equivalent/ml) in the control group, respectively. However, the value of undernourished group showed decreases compared with those in the control group (70.3,46.8 and 98.5% of each opposite control). The mucosal uptake amount of RI labeled BSA at 9 and 16 days were increased significantly in the undernourished group (5.6,1.9 times of control), but the amount of that at 23 days was not significantly defferent from control. On the other hand, that of RI labeled BLG was more increased compared with each opposite control (69.6,2.3,3.4 times). The Cathepsin B activity (U/mg protein/ml) of the control liver at 9,16,23 days showed 10.4,18.1,9.7. The activity of the undernourished group was significantly declined on these days (7.9,10.0,7.1). While, that of intestinal mucosa groups showed significant decreases compared with those in control group at 9 and 16 days (4.4,4.3), but significant increases (2 times the control value) at 23 days.These studies suggest that neonatal undernutrition may have unfavorable influences on the subsequent development of protein digestive and absorptive function, and may be a factor in development of future allergic disease.

我们已经检查了新生儿不足对大鼠肠道蛋白质脱位和吸收功能的影响。对照组的一个垃圾饲养了8只幼崽，而营养不良的组中的16只幼崽在2岁开始饲养1周，然后将8只小狗作为垃圾饲养。肠道内腔（蛋白水解活性），蛋白质吸收功能（皮细胞活性）的蛋白质消化功能，通过使用过肠sac和肠粘膜和肝脏的肠粘膜的活性在2,9,16和23天的对照和不足的pups中测量。对照组中的等效/ml）。然而，与对照组相比，营养不良组的值显示出降低（每个相反对照的70.3,46.8和98.5％）。在营养不良的组中，在9和16天后标记为BSA的RI的粘膜摄取量显着增加（控制的5.6,1.9倍），但是在23天时，该组的含量并未明显与对照相比。另一方面，与每个相反的对照相比，标记为BLG的RI的增加（69.6,2.3,4倍）。在9,16,23天，对照肝的组织蛋白酶B活性（U/mg蛋白/mL）的活性为10.4,18.1,9.7。如今，营养不良组的活性显着下降（7.9,10.0,7.1）。而与对照组的9天和16天（4.4,4.3）相比，肠粘膜组的肠道粘膜组显示出显着降低，但在23天时，显着增加（对照值的2倍）。这些研究表明，新生儿不足的营养不良可能对蛋白质消化和吸收性功能的后续发展可能会产生不利的影响，并且可能是一种因素，并且可能是一种未来的疾病。