Microwave-Enhanced Microreactor for Ultrafast Detection of Protein Biomarkers

用于超快速检测蛋白质生物标志物的微波增强微反应器

基本信息

批准号：
10483753
负责人：
Steven Ray
金额：
$ 25.99万
依托单位：
VIC FOUNDRY, INC.
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-09-01 至 2024-02-29
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10483753
关键词：
Acute Address Antibody Specificity Biological Biological Markers Buffaloes C-reactive protein COVID-19 patient COVID-19 severity Cardiac Characteristics Clinical Coupled Coupling Creatine Kinase Critical Illness Detection Diagnosis Digestion Disease Drug Targeting Electrospray Ionization Glial Fibrillary Acidic Protein Goals Heart Heating Immunoassay In Situ Infection Inflammatory Interleukin-6 Interleukin-8 Legal patent Life Mass Spectrum Analysis Modeling Monitor Myocardial Infarction Myoglobin Patients Peptides Performance Phase Preparation Process Protein Analysis Proteins Reaction Research S100 Calcium Binding Protein Sampling Sepsis Serum Site Small Business Technology Transfer Research System TNF gene Technology Testing Therapeutic Time Traumatic Brain Injury Troponin I Trypsin Tube Universities absorption acute coronary syndrome antibody conjugate base clinical diagnosis cytokine design detection limit detector engineering design fatty acid-binding proteins immunoreaction inflammatory marker ionization liquid chromatography mass spectrometry magnetic beads microwave electromagnetic radiation nano-electrospray photomultiplier pilot test procalcitonin prognostication protein B protein aminoacid sequence protein biomarkers prototype risk stratification specific biomarkers ubiquitin C-terminal hydrolase

项目摘要

Project Summary In diagnosis, risk stratification, and management of life-threatening diseases/conditions, every minute counts and unambiguous detection of many relevant protein biomarkers is crucial. Unfortunately, the workflow of current unambiguous approach–mass spectrometry–is bottlenecked by the sample preparation process. A microwave-enhanced, flow-through microreactor is proposed here for both selective capture and digestion of proteins to address this major limitation. The specific aims of this Phase I STTR are to create a prototype microreactor and to demonstrate a proof of concept of the microreactor-enabled mass spectrometry analysis of protein biomarkers. The proposed technology and product will streamline the workflow of mass spectrometry analysis of proteins, free end users from the laborious and tedious multiple sample preparation steps, and allow near real-time and unambiguous detection of protein biomarkers, as well as therapeutics, drug targets, etc.

项目概要在危及生命的疾病/状况的诊断、风险分层和管理中，每个分钟计数和许多相关蛋白质生物标志物的明确检测至关重要。不幸的是，当前明确的方法——质谱——的工作流程是微波增强的流通过程成为瓶颈。这里提出了微反应器用于蛋白质的选择性捕获和消化，以解决这一主要限制是第一阶段 STTR 的具体目标是创建一个原型。微反应器并展示微反应器启用质量的概念证明蛋白质生物标志物的光谱分析。所提出的技术和产品将简化质谱分析的工作流程蛋白质分析，使最终用户免于繁琐的多次样品制备步骤，并允许近乎实时和明确地检测蛋白质生物标志物，以及治疗方法、药物靶点等