VITAMIN E REQUIREMENT OF SEAWATER FISH LARVAE

海水鱼幼虫对维生素E的需求

基本信息

批准号：
06660258
负责人：
TAKEUCHI Toshio
金额：
$ 1.34万
依托单位：
TOKYO UNIVERSITY OF FISHERIES
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06660258/
关键词：
vitamin E yellowtail pacific cod larvae docosahexaenoic acid rotifer

项目摘要

This research is conducted to investigate the absorption of vitamin E (VE) in rotifer and seawater fish larvae such as yellowtail and Pacific cod. Different kinds of VE,such as free form and acetate form were used in this experiment.At first, the accumulation of free-and acetate-form of VE was investigated using rotifer. We obtained linear curves as follows ; Free form of VE : y_1=0.001X_1^2+0.026X_1+0.435 (r^2=0.996) Acetate form of VE : y_2=0.077X_2+0.078 (r^2=0.966) y_<1,2>=VE content (mg) of rotifer, X_<1,2>=supplementation of VE (mg) to rotifer culture medium. This result showed that free-and acetate-form are no different in accumulation till 100mg/101 of VE supplementation. However, at a level of above 100mg of VE supplementation, free type of VE is more incorporated into rotifer than acetate type.Next, dl-acetate, dl-free, and d-free forms of VE were added to rotifer medium, and yellowtail and pacific cod larvae fed these enriched rotifers. It was difficult for rotifer to convert acetate form to free form. Fish larvae can convert acetate form to be a free form of VE,but its absorption was low compared with free form. However, growth and survival rate of fish larvae were not different between these kinds of VE form.Yuglena was also used for emulsified materials. It is suggested that VE in Yuglena did not accumulate in the body of the fish very much.These observations are useful when considering the design of emulsified materials for live foods and micro-diet for fish larvae.

本研究旨在调查轮虫和海水鱼幼虫（例如黄尾鱼和太平洋鳕鱼）对维生素 E (VE) 的吸收。本实验使用了不同种类的VE，如游离型和醋酸型VE。首先，利用轮虫研究了游离型和醋酸型VE的积累。我们得到的线性曲线如下； VE 的自由形式： y_1=0.001X_1^2+0.026X_1+0.435 (r^2=0.996) VE 的乙酸酯形式： y_2=0.077X_2+0.078 (r^2=0.966) y_<1,2>=VE 含量(毫克)轮虫， X_<1,2>=轮虫培养基中VE的补充量(mg)。该结果表明，在补充 100mg/101 VE 之前，游离形式和乙酸酯形式的积累没有差异。然而，在 VE 补充水平高于 100mg 时，游离型 VE 比醋酸型 VE 更多地融入轮虫中。接下来，将 dl-醋酸盐、无 dl 和无 dl 形式的 VE 添加到轮虫培养基中，并在黄尾鱼培养基中添加 VE。太平洋鳕鱼幼虫以这些丰富的轮虫为食。轮虫很难将乙酸盐形式转化为游离形式。鱼类幼虫可将乙酸盐形式转化为游离形式的VE，但其吸收量较游离形式低。然而，这些VE形式的鱼幼体的生长和存活率没有差异。尤格莱纳也被用于乳化材料。表明玉藻中的VE在鱼体内的积累并不多。这些观察结果对于考虑活食乳化材料和鱼幼体微量饲料的设计是有用的。