Studies of the localization and distribution of the trigeminal nerve fibres and SP- and CGRP-containing nerve fibres in rat gingivae with experimental gingivitis or without gingivit

实验性牙龈炎或无牙龈大鼠牙龈中三叉神经纤维和含 SP 和 CGRP 的神经纤维的定位和分布研究

基本信息

批准号：
02807166
负责人：
TANAKA Teruo
金额：
$ 1.02万
依托单位：
Kyushu University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1991
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02807166/
关键词：
junctional epithelium WGA-HRP substance P CGRP immunohistochemistry

项目摘要

The sensory innervation of junctional and oral sulcular epithelium was investigated by use of anterograde transport of wheat-germ agglutinin-horseradish peroxidase from the trigeminal ganglion in rats. By light microscopy, labeled intraepithelial nerve fibers were observed forming a dense plexus in the apical two-thirds of the junctional epithelium. In contrast to the junctional epithelium, the oral sulcular epithelium was sparsely innervated. By electron microscopy, labeled intraiepithelial axon terminals or varicosities were demonstrated to be in close contact both with junctional epithelial cells and neutrophils. These varicosities frequently contained large granular and small clear vesicles.The beginning of nerve fibers with Substance P(SP)- or calcitonin gene-related peptide like immunoreactivity(CGRP-IR)in the developing junctional epithelium was examined by the avidin-biotincomplex-method. - In 21-day old rats, varicose fibers were first entered the coronal and middle portions of reduced enamel epithelium. In 23-day-old rats, many fibers with SP- or CGRP-IR were seen in the basal layers, but only a few in the superficial layers of the junctional epithelium. In 28 to 32-day-old rats, numerous fibers with SP- or CGRP-IR were distributed in the whole junctional epithelium.Nerve fibers with SP- or CGRP-IR in the labial gingival tissues of mandibular incisors affected with gingivitis and without gingivitis were observed. In the gingivitis affected rats, the fibers showed a marked increase in number and density compared with the normal rats ; many of them were located close to blood vessels. At the enamel side of the periosteum, many nerve fibers with SP- or CGRP-IR were located around the vessels and some fibers were close to the alveolar bone, but few were found along the oral epithelial side of the periosteum.

通过利用大鼠三叉神经节的麦芽凝集素-辣根过氧化物酶的顺行转运来研究交界和口腔龈沟上皮的感觉神经支配。通过光学显微镜，观察到标记的上皮内神经纤维在交界上皮的三分之二的顶端形成致密丛。与交界上皮相反，口腔沟上皮神经稀疏。通过电子显微镜，标记的上皮内轴突末端或静脉曲张被证明与交界上皮细胞和中性粒细胞紧密接触。这些静脉曲张经常含有大颗粒和小透明囊泡。通过亲和素-生物素复合物方法检查发育中的交界上皮中具有 P(SP) 物质或降钙素基因相关肽样免疫反应性 (CGRP-IR) 的神经纤维的起点。 - 在21天龄的大鼠中，曲张纤维首先进入减少的牙釉质上皮的冠状部和中部。在23日龄的大鼠中，在基底层中可见到许多带有SP-或CGRP-IR的纤维，但在交界上皮的浅层中仅见少量。 28～32日龄大鼠，整个交界上皮中分布有大量带有SP-或CGRP-IR的神经纤维。有牙龈炎和无牙龈炎的下颌切牙唇龈组织中带有SP-或CGRP-IR的神经纤维被观察到。在患有牙龈炎的大鼠中，与正常大鼠相比，纤维的数量和密度明显增加；其中许多位于血管附近。在骨膜的釉质侧，许多带有SP-或CGRP-IR的神经纤维位于血管周围，一些纤维靠近牙槽骨，但沿着骨膜的口腔上皮侧发现很少。