Expression and production of proteins containing in hemidesmosme/besement membrane at the interface between the implant-oral epithelium.

种植体-口腔上皮之间界面处的半桥粒/基膜中含有的蛋白质的表达和产生。

• 批准号: 13470385
• 负责人: TANAKA Teruo
• 金额: $ 5.89万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13470385/
• 关键词: titanium; oral epithelial cells; cell attachment; hemidesmosome; basement menbrane; laminin-5; PI3-kinase; F-アクチン; IGF; インプラント; ラミニン5; 免疫細胞科学; 歯肉; 接合上皮; ラット; 細胞培養; 免疫細胞化学

Laminin-5(Ln-5), which is associated with epithelial cell adhesion and migration, is present in the internal and external basement laminas(IBL and EBL) of the junctional epithelium(JE), whereas the detailed localization of Ln-5 in the peri-implant epithelium(PIE) remains unclear. By immuno-light microscopy, the immunoreactive product of Ln-5 appeared as a band along the implant-PIE interface, while most of the upper portion of the interface did not express Ln-5. Ln-5 was expressed along the connective tissue interfaces with the PIE, peri-implant sulcular epithelium, and oral epithelium. Immuno-electron microscopy showed that Ln-5 was expressed in the cells of the innermost-third layer and basal layer of the PIE, and in the IBL and EBL of the PIE. It was characteristic that a 100-nm-wide Ln-5-positive IBL and hemidesmosomes(HDs) were formed only in the lower portion of the interface. In addition, Ln-5-positive EBL was formed at most PIE-connective tissue interface, although at some part … More s of the interface, EBL and HDs were not formed. Next, we investigated the distribution of Ln-5 during the formation of PIE. One day after extraction, the JE had disappeared. After 3 days, Ln-5-positive epithelial cells extended horizontally over the wound at the leading edge from the oral sulcular epithelium(OSE). After 5 days, the epithelium extending from the OSE on each side of the wound joined and formed additional new epithelium. The new epithelium expressed Ln-5 in the BM. After 1 to 2 weeks, the oral epithelium extending from the sides of the wound joined in the center. Three days after implantation, no JE remained. Ln-5-positive epithelial cells extended apically from the OSE. After 1 to 2 weeks, the PIE was formed. Ln-5 was expressed at the PIE-connective tissue interface. Finally, after 4 weeks, Ln-5 was expressed at the implant-PIE interface, and the PIE was non-keratinized epithelium. Our findings suggest that partial deficiency of the IBL,EBL,and HDs in the PIE is associated with the presence of Ln-5, which is required for the formation of these attachment apparatuses, and that the PIE is a poorly adherent, permeable epithelium because of the partial deficiency of the attachment apparatuses, as compared to the JE. Furthermore it is suggested that Ln-5 induces cell migration during PIE formation, and that PIE originates from OSE. Less

层粘连蛋白-5（Ln-5）与上皮细胞粘附和迁移相关，存在于交界上皮（JE）的内部和外部基底层（IBL和EBL）中，而Ln-5的详细定位在种植体周围上皮 (PIE) 仍不清楚，通过免疫光学显微镜，Ln-5 的免疫反应产物出现为沿种植体 PIE 的条带。界面上部不表达 Ln-5，而 PIE、种植体周围龈沟上皮和口腔上皮的结缔组织界面则表达 Ln-5。 5在PIE的最内层第三层和基底层的细胞中表达，并且在PIE的IBL和EBL中表达。 100 nm宽的Ln-5阳性IBL和半桥粒(HD)仅在界面的下部形成。此外，尽管在某些部分，但在大多数PIE-结缔组织界面处形成Ln-5阳性EBL。 … 更多界面、EBL 和 HD 均未形成。 接下来，我们研究了 PIE 形成过程中的 Ln-5 分布，3 天后，JE 消失了。 Ln-5阳性上皮细胞从口腔沟上皮（OSE）的前缘水平延伸到伤口上。5天后，从伤口两侧的OSE延伸的上皮连接并形成额外的新上皮。 1至2周后，从伤口两侧延伸的口腔上皮在BM中表达Ln-5，植入后三天，没有。 1至2周后，Ln-5阳性上皮细胞在PIE-结缔组织界面处表达。在种植体-PIE 界面上表达，并且 PIE 是非角化上皮，我们的研究结果表明 PIE 中 IBL、EBL 和 HD 的部分缺陷与 PIE 的存在有关。 Ln-5，这是形成这些附着装置所必需的，并且与 JE 相比，由于附着装置的部分缺陷，PIE 是粘附性差、渗透性好的上皮。 此外，建议使用 Ln-5。在 PIE 形成过程中诱导细胞迁移，并且 PIE 源自 OSE Less。

项目成果

Kajiwara H, Yamaza T, Yoshinari M, Goto T, Iyama S, Atsuta I, Kido MA, Tanaka T.: "The bispohosphonate pamidronate on the surface of titanium stimulates bone formation around tibial implants in rats"Biomaterials. In press.

Kajiwara H、Yamaza T、Yoshinari M、Goto T、Iyama S、Atsuta I、Kido MA、Tanaka T.：“钛表面上的双磷膦酸帕米膦酸盐刺激大鼠胫骨植入物周围的骨形成”生物材料。

田中 輝男: "ザ・クインテッセンスVol.21 no.1"クインテッセンス出版. 11 (2002)

田中辉雄：《精粹 Vol.21 no.1》精粹出版社 11 (2002)。

Ikiru Atsuta, Takayoshi Yamaza, Masao Yoshinari, Satoya Mino, Tetsuya Goto, Mizuho A Kido, Yoshihiro Terada, Teruo Tanaka: "Changes in the Distribution of Laminin-5 during Peri-Implant Formation after Immediate Titanium Implantation in Rats"Biomaterials.

Ikiru Atsuta、Takayoshi Yamaza、Masao Yoshinari、Satoya Mino、Tetsuya Goto、Mizuho A Kido、Yoshihiro Terada、Teruo Tanaka：“大鼠即刻钛植入后植入物周围形成过程中 Laminin-5 分布的变化”生物材料。

Kajiwara H, Yamaza T, Yoshinari M, Goto T, Iyama S, Atsuta I, Kido MA, Tanaka T: "The bispohosphonate pamidronate on the surface of titanium stimulates bone formation around tibial implants in rats"Biomaterials. (In press).

Kajiwara H、Yamaza T、Yoshinari M、Goto T、Iyama S、Atsuta I、Kido MA、Tanaka T：“钛表面的双磷酸盐帕米膦酸盐刺激大鼠胫骨植入物周围的骨形成”生物材料。

Ikiru Atsuta, Takayoshi Yamaza, Masao Yoshinari, Satoya Mino, Tetsuya Goto, Mizuho A Kido, Yoshihiro Terada, Teruo Tanaka: "Changes in the Distribution of Laminin-5 during Peri-Implant Epithelium Formation after Immediate Titanium Implantation in Rats."Bi

Ikiru Atsuta、Takayoshi Yamaza、Masao Yoshinari、Satoya Mino、Tetsuya Goto、Mizuho A Kido、Yoshihiro Terada、Teruo Tanaka：“大鼠即刻钛植入后种植体周围上皮形成期间层粘连蛋白 5 的分布变化。”Bi