Study on signal transduction and involvement of protein synthesis machinery in the production of Bacilhus subtilis proteass

枯草芽孢杆菌蛋白酶生产中信号转导及蛋白质合成机制的研究

基本信息

批准号：
11460050
负责人：
TANAKA Teruo
金额：
$ 9.6万
依托单位：
TOKAI UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11460050/
关键词：
Bacillus subtilis two-c-mp0nent regulatory system DegS-DegU lincomycin aprE expression stringgent facotr ReIA microarray 細胞外分解酵素アルカリプロタアーゼタンパク合成阻害物質 Stringent factor RelA ppGpp リン酸化DegU アルカリプロテアーゼ stringent factor RelA 菌体

项目摘要

Microorganisms in nature show various modes of life style. Bacillus subtilis is a soil bacterium that encounters severe environmental stresses such as nutritional deficiency, desiccation, drastic temperature changes and so on. The bacterium, however, carries various means to cope with these unfavorable situations. Against nutritional deficiency, for example, it secretes proteases and degradative enzymes to digest high molecular weight proteins or carbohydrates that happen to be present around the cell, and takes up the hydrolyzed products for nutrition. The machinery that serves to sense the environment is thought to be the two-component regulatory system in which the sensor kinase detect environmental cues and phosphorylates the cognate regulator, which then activates the target genes.We have studied a signal transduction system involving a two-component system, DegS-DegU, which regulates the production of extracellular degradative enzymes. For the investigation of cellular processes … More in general, the most useful approach will be to use genetic means. For the DegS-DegU system, however, many genetic approaches have already been applied, and therefore the possibility to obtain new findings seemed to be low. We tried, therefore, to find chemical agents that might affect the signal transduction involving DegS-DegU. As a result, antibiotics such as lincomycin and erythromycin that are known to inhibit protein biosynthesis through affecting the ribosomal function were found to prevent the expression of the alkaline protease gene, aprE, the target of the DegS-DegU system. In this study we tried to identify the target of the antibiotics through which the DegS-DegU transmits a signal to induce the expression of aprE. We found that lincomycin inhibits RelA-dependent ppGpp synthesis. Furthermore, the expression of aprE was greatly reduced in relA-deficient cell. These results indicate that lincomycin prevents aprE expression by inhibiting ppGpp synthesis. This is the first report that an antibiotic inhibits signal transduction.We also applied microarray analysis on B. subtilis two-component systems that carry a helix-tum-helix motif in the regulator, and found that networks are formed among several of the systems. Less

自然界中的微生物显示了各种生活方式，较大的温度变化等等。在未及时成为靶基因的tweconment系统中，我们研究了涉及两个组件系统DEGS-DEGU的信号转导系统，该系统调节了细胞外降解酶的产生。蜂窝过程...在GEGU系统中，更多的IC表示，已经应用了许多遗传方法，因此，获得新发现的可能性似乎很低。林霉素和红霉素等依霉素抑制蛋白质生物合成的核糖体融合型融合功能被发现是碱性蛋白酶基因的表达，这是degu系统的靶标。。那是在几个系统中形成的