Study on possible sympathetic modulation of dental pain

牙痛可能的交感调节研究

• 批准号: 21791836
• 负责人: SHIMENO Yoichi
• 金额: $ 2万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21791836/
• 关键词: 交感神経依存性疼痛; 歯痛; 末梢修飾機序; ＷＧＡ－ＨＲＰ; 交感神経; 炎症歯髄; 発芽; ラット; WGA-HRP

This study was designed to determine if sympathetic nerve fibers exist in dentinal tubules in rat normal dental pulp, and if they sprout into the dentinal tubules in response to artificial cavity preparation in dentin. Sympathetic nerve fibers in rat molar dental pulp were labeled using an anterograde axonal transport technique involving injection of wheat germ agglutinin-horseradish peroxidase (WGA-HRP) into the superior cervical ganglion (SCG). They were then observed using light and electron microscopes. In normal dental pulp (control), scattered WGA-HRP reaction products were observed in unmyelinated nerve endings in the odontoblast layer and subodontoblastic region. In injured pulp 3 weeks after cavity preparation, reaction products were about1.8-times more plentiful in the above areas (versus control pulp). However, no labeled nerve fibers were observed in the dentinal tubules in either control or injured dental pulp. These results indicate that although sympathetic nerve fibers do indeed sprout in rat dental pulp in response to cavity preparation, they do not penetrate into the dentinal tubules in which postganglionic nerve endings derived from the SCG were not originally present.

本研究旨在确定大鼠正常牙髓的牙本质小管中是否存在交感神经纤维，以及交感神经纤维是否响应牙本质中的人工空腔制备而萌芽到牙本质小管中。使用顺行轴突运输技术标记大鼠磨牙牙髓中的交感神经纤维，该技术涉及将麦芽凝集素-辣根过氧化物酶（WGA-HRP）注射到上颈神经节（SCG）中。然后使用光学显微镜和电子显微镜观察它们。在正常牙髓（对照）中，在成牙本质细胞层和牙本质细胞下区域的无髓鞘神经末梢中观察到分散的WGA-HRP反应产物。在空腔准备后3周的损伤牙髓中，上述区域的反应产物大约是对照牙髓的1.8倍。然而，在对照或受损牙髓的牙本质小管中没有观察到标记的神经纤维。这些结果表明，尽管交感神经纤维确实在大鼠牙髓中响应空洞准备而发芽，但它们不会渗透到牙本质小管中，而牙本质小管中最初不存在源自 SCG 的节后神经末梢。

项目成果

歯痛に関する診断学的研究-歯痛に及ぼす交感神経の影響について-

牙痛的诊断研究-关于交感神经对牙痛的影响-

• 发表时间: 2011
• 作者: Watanabe S;Kokuzawa C;Gomb B;Saegusa H;AnjoT;Ebihara A;Kobayashi C;Suda H;庄司憲明,示野陽一,菅原由美子,飯久保正弘,佐藤しづ子,笹野高嗣
• 通讯作者: 庄司憲明,示野陽一,菅原由美子,飯久保正弘,佐藤しづ子,笹野高嗣