Study of The Mechanism of Prolactin Cell Differentiation in The Anterior Pituitary

垂体前叶催乳素细胞分化机制的研究

基本信息

批准号：
02807001
负责人：
INOUE Kinji
金额：
$ 1.02万
依托单位：
Gunma University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1991
项目状态：
已结题

项目摘要

Newly established MtT/S-secrete only the rowth hormone(GH). Electron microscopic examination of the MTT/S cells showed a normal somatotroph-like appearance i. e. ., the presence of many growth hormone containing secretory granules, well developed endoplasmic reticulum and Golgi apparatus in their cytoplasm. GRH stimulate GH secretion of MtT/S cell line at the plateau-phase of cell growth. Interestingly, the MTT/S cells in the mitotic phase also contained secretory granules. Furthermore, all of the bromo-deoxyuridine(BrdU)incorporated cells which known an indicator of proliferative cells are growth hormone cells. These characteristics of MTT/S cells are showing this cells proliferate as the GH-cells. No' prolactin(PRL)-cells were observed among MTT/S cells cultured in regular medium, and no PRL in medium and cells was detected with radioimmunoassy and Western blotting analyses, whereas insulin or insulin-like growth factor-1(IGF-1)exerted a marked decrease-in. GH-production and GH-cell … More population. In the medium PRL was detected after a 2-day lag period and a numerous PRecells appeared 5 days after stimulation with insulin or IGF-1. Double immunocytochemistry clearly indicated that no cell contained both PRL and GH. These results show that PRecell transdifferentiated from GH-cells by stimulation with insulin or IGF-1 and this suggests that the MTT/S cell line is an excellent model system showing GHcell/PRecell linea, e. Recent information of the molecular biology indicated that the mammotroph in pituitary cell may-differentiate from somatotroph. However, intra- or extra-cellular mechanism regulating cell differentiation of mammotroph from somatotroph remain unclear. Our newly established MTT/S cell line seems good model for reveal the mechanism pituitary cell differentiation.On the other hand, by the addition of EGF with insulin, the transdifferentiation of GH cells to prolactin is very accelerated and somatomammotroph which have both GH and prolactin were appeared. The existence of somatomammotroph in the pituitary gland is well known however, the meaning of the somatomammotroph in the pituitary gland are unclear. Our result of the appearance of somatomammotroph by the stimulation of insulin and EGF may show somatomammotroph appeared on the process of transdifferentiation of GH-cells to prolactin cells. Less

新成立的MTT/S-Secrete仅Rowth Horsene（GH）。 MTT/S细胞的电子显微镜检查表现出正常的类型体形样外观i。 e。。 GRH在细胞生长的平台期刺激MTT/S细胞系的GH分泌。有趣的是，有丝分裂阶段的MTT/S细胞还包含秘密颗粒。此外，所有的Bromo-脱氧尿苷（BRDU）掺入了所有知道增殖细胞指标的细胞都在生长赛酮细胞。 MTT/S细胞的这些特征表明该细胞随着GH细胞的形式增殖。在常规培养基中培养的MTT/S细胞中未观察到催乳素（PRL） - 细胞，并且在培养基中没有PRL，并且通过放射免疫和蛋白质印迹分析检测细胞，而细胞则检测到细胞，而胰岛素或胰岛素样生长因子-1（IGF-1）则施加了明显的降低。 GH产生和GH-Cell…更多的人口。在2天的滞后周期后检测到培养基中的PRL，并在用胰岛素或IGF-1刺激后5天出现了许多预售。双重免疫细胞化学清楚地表明，没有细胞同时包含PRL和GH。这些结果表明，用胰岛素或IGF-1刺激从GH细胞转分化的predell脱节，这表明MTT/S细胞系是一个出色的模型系统，显示了GHCELL/PRECELL LILEA，E。分子生物学的最新信息表明，垂体细胞中的乳腺营养物可能与人体营养不良。然而，对乳腺营养的细胞分化的细胞内机制或细胞外机制尚不清楚。我们新建立的MTT/S细胞系似乎是揭示垂体细胞分化机制的好模型。另一方面，通过将EGF添加到胰岛素中，GH细胞向催乳素的转分化非常加速，并且会出现GH和催乳素。垂体中的体瘤存在在垂体中众所周知，垂体腺体营养不良的含义尚不清楚。我们通过刺激胰岛素和EGF出现躯体营养的结果可能显示出在GH细胞向催乳素细胞转分解的过程中出现体营养素。较少的