Study on the function of folliculo-stellate cells in the anterior pituitary gland, especially the analysis of their secretory protein.

垂体前叶滤泡星状细胞功能的研究，特别是其分泌蛋白的分析。

基本信息

批准号：
08457257
负责人：
INOUE Kinji
金额：
$ 1.73万
依托单位：
Saitama University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1998
项目状态：
已结题

项目摘要

The anterior pituitary gland is composed of endocrine cells and supportive cells. The folliculo-stellate cell(FS-cell) is the major cellular element of these supportive cells and expected that ES-cells have very important function in the anterior pituitary gland. However, the precise function of FS cells in the anterior pituitary gland has not been established. The purpose of this research project is a study of the function of folliculo stellate cells. We first noticed the appearance of large number of PAS positive colloid in the senescent porcine pituitary gland Our morphological study revealed that these PAS positive colloids were completely surrounded by the FS cells. Therefore, the characterization of the colloidal substance may be important for the analysis of FS cell function. Fortunately we have succeeded in purifying and analyzing the collois using the porcine pituitary gland and revealed the colloids contained large amount of clusterin. The clusterin is known as the highly glycosilated protein and suspected that it may be related to the neural cell apoptosis in the brain. Our immunocytochemical study showed clusterin is not produced by FS cells but some endocrine cells. These results suggest the cells produced chusterin were undergone to the apoptosis and phagocytosed by the FS cells and the clusterin accumulated in the colloid as the residual body. The colloid appearance in the pituitary gland is well known phenomenon however this is the first study which revealed the chemical component of those colloids, For the analysis the biological significance of the clusterin appearance in the pituitary gland is need further study.

垂体前腺由内分泌细胞和支撑性细胞组成。 Folliculo-Stellate细胞（FS细胞）是这些支撑性细胞的主要细胞元素，并期望ES细胞在垂体前腺具有非常重要的功能。但是，尚未建立FS细胞在垂体前腺体中的精确功能。该研究项目的目的是研究Folliculo stellate细胞的功能。我们首先注意到在衰老猪垂体中出现大量PAS阳性胶体，我们的形态学研究表明，这些PAS阳性胶体完全被FS细胞包围。因此，胶体物质的表征对于分析FS细胞功能可能很重要。幸运的是，我们已经成功地使用猪垂体纯化和分析了高素质，并揭示了胶体中含有大量簇蛋白。簇蛋白被称为高糖化蛋白，并怀疑它可能与大脑的神经细胞凋亡有关。我们的免疫细胞化学研究表明，簇蛋白不是由FS细胞而是某些内分泌细胞产生的。这些结果表明，产生的曲藻蛋白的细胞经历了凋亡，并由FS细胞吞噬，簇蛋白积聚在胶体中作为残留体。垂体中的胶体外观是众所周知的现象，但是这是第一个揭示这些胶体的化学成分的研究，以分析垂体中簇蛋白出现的生物学意义，需要进一步研究。