Analysis of paracrine regulation of endocrine gland function

内分泌腺功能的旁分泌调节分析

• 批准号: 08044246
• 负责人: INOUE Kinji
• 金额: $ 1.66万
• 依托单位: Saitama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for international Scientific Research
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08044246/
• 关键词: anterior pituitary gland; paracrine; cytokine; M-CSF; PACAP; LPS; Prolactin; macrophage; バラクリン

The anterior pituitary gland cells produce and secrete at least five kinds of hormones which are important for the regulation of growth, metabolism, reproduction and immunity. It is well known that the anterior pituitary glandular cells are regulated by hypothalamic factors. The anterior pituitary glandular cells also respond to some physiological changes such as the estrogen treatment and castration etc. and undergo hypertrophy and hyperplasia. These later dynamic changes of the anterior pituitary gland cells however can not be explained solely by hypothalamic factors. It has been postulated the paracrine regulation may be important for the regulation of the secretory cells. The folliculo-stellate cells which surround neighboring secretory cells with their long cytoplasmic processes are considered as the most important cells for the pracrine regulation of the glandular cells. In this research we found that the FS cells produce IL-8 and macrophage colony stimulating factors under the regulation of a hypotharamic factor, PACAP.The production of these cytokines was significantly increased by the stimulation of lipopolysaccharide (LPS). LPS also stimulates the secretion of prolactin cramatically. These results suggest that the cytokines, which are known as the signals of the immune system, are controled to some degree by hormone-secreting pituitary cells through paracrine regulation.

垂体前腺细胞至少产生并分泌五种激素，这对于调节生长，代谢，繁殖和免疫力很重要。众所周知，垂体前腺细胞受到下丘脑因子的调节。垂体前腺细胞也应对某些生理变化，例如雌激素治疗和cast割等，并经历肥大和增生。但是，这些后来的垂体前腺细胞的动态变化不能完全由下丘脑因素来解释。已经假定旁分泌调节对于分泌细胞的调节可能很重要。围绕附近分泌细胞及其长的细胞质过程的Folliculo-Stellate细胞被认为是腺细胞pracrine调节的最重要细胞。在这项研究中，我们发现FS细胞在下丘脑因子调节下产生IL-8和巨噬细胞刺激因子，PACAP。这些细胞因子的产生通过刺激脂多糖（LPS）显着增加。 LPS还会刺激性地刺激催乳素的分泌。这些结果表明，被称为免疫系统信号的细胞因子通过通过旁分泌调节来控制垂体细胞在某种程度上控制一定程度。

项目成果

S.Tanaka et al.: "Proteolitic processing of proopiomeranocortin occures in acidifiing secretory granules of AtT-20 cells." J.Histochem.Cytochem. (in press). (1997)

S.Tanaka 等人：“阿匹皮素原的蛋白水解过程发生在 AtT-20 细胞的酸化分泌颗粒中。”

H.Nogami, K.Inoue and K.K.Kawamura: "The mechanism of induction of hormone expression by glucocorticoid in the fetal pituitary gland in vitro" Endocrinology. (in press). (1997)

H.Nogami、K.Inoue 和 K.K.Kawamura：“体外胎儿垂体中糖皮质激素诱导激素表达的机制”内分泌学。

S.Ogawa et al.: "The glucoprotins that occur in the colloids of senescent porcine pituitary glands are clusterin and glycosylated alubumin." BBRC.(submitted).

S.Okawa 等人：“衰老猪脑下垂体胶体中存在的葡糖蛋白是簇蛋白和糖基化白蛋白。”