MECHANISM OF CELL DIFFERENTIATION OF ENDOCRINE CELL, WITH SPECIAL REFERENCE TO THE SOMATOMAMMOTROPH IN ANTERIOR PITUITARY GLAND.

内分泌细胞的细胞分化机制，特别是垂体前叶的生长发育细胞。

基本信息

批准号：
04670003
负责人：
INOUE Kinji
金额：
$ 0.58万
依托单位：
FACULTY OF SCIENCE OF SAITAMA UNIVERSITY (1993)Gunma University (1992)
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1993
项目状态：
已结题

项目摘要

The endocrine gland, such as an anterior gland consist from the mixed population of different hormone secreting glandular cells. These different glandular cells supposedly differentiated from same stem cell however, the mechanism of cell differentiation in endocrine cells still under discusson. We recently established cell line(MtT/S)from rat plaractinoma. The MtT/S cell line produces only growth hormone(GH)in normal growth medium. The MtT/S cells labeled with bromodeoxyuridine(BrdU)also show staining for GH.Furthermore, mitotic MtT/S cells contain GH immunopositive secretory granules in their cytoplasm. These features indicate that MtT/D cells proliferate as GH-secreting cells. We reported previouly that MtT/S cells transdifferentiate into prolactin cells upon stimulation with insulin or insulin-like growth factor. A requirement of estrogen priming for MtT/S cell transdifferentiation has also been reported. This transdifferentiation is considerably accelerated by addition of EGF, resulting in somatomammotrophs contain both prolactin and GH.In order to reveal the relationship between cell proliferation and cell differentiation, a BrdU labeling experiment was performed. the results indicated that prolactin cells which were induced by stimulation with insulin and EGF showed markedly suppressed BrdU incorporation, indicating that prolactin cells that have transdifferentiated from growth hormone cells are non-proliferate or show only very slow proliferation. In general, prolactin cells are known to be the most active proliferative cells in the pituitary gland. This discrepancy between in vitro and in vivo result is verly important, since it may indicate an unknown mechanism for the regulation of prolacrtin cell proliferation in the anterior pituitary gland. On the other hand these cell differentiation may be controlled by neighboring supportive cells through cell cell communication. The folliculo-stellate cells in the anterior pituitary gland is the most important cand

内分泌腺，例如前腺由不同激素分泌细胞的混合种群组成。这些不同的腺细胞据说与同一干细胞区分开，但是，内分泌细胞中细胞分化的机理仍在讨论下。我们最近从大鼠plaractinoma建立了细胞系（MTT/S）。 MTT/S细胞系仅在正常生长培养基中产生生长激素（GH）。标记为溴脱氧尿苷（BRDU）的MTT/S细胞还显示了GH.Furthermore的染色，有丝分裂MTT/S细胞在其细胞质中含有GH免疫阳性分泌颗粒。这些特征表明MTT/D细胞随着GH分泌细胞的形式增殖。我们报告说，用胰岛素或胰岛素样生长因子刺激后，MTT/S细胞在催乳素细胞中转分化。还报道了雌激素启动对MTT/S细胞转分化的要求。通过添加EGF，可以大大加速这种转化，从而导致人体营养素含有催乳素和GH。为了揭示细胞增殖与细胞分化之间的关系，进行了BRDU标记实验。结果表明，用胰岛素和EGF刺激诱导的催乳素细胞表现出明显抑制的BRDU掺入，表明从生长激素细胞转差的催乳素细胞非促分化或仅显示非常缓慢的增殖。通常，已知催乳素细胞是垂体中最活跃的增殖细胞。体外和体内结果之间的这种差异非常重要，因为它可能表明在垂体前垂体中调节prop蛋白细胞增殖的未知机制。另一方面，这些细胞分化可以通过细胞通信来控制相邻的支持细胞。垂体前腺中的Folliculo stellate细胞是最重要的CAND