Studies on toxification of puffer fish.

河豚鱼毒性研究.

• 批准号: 63560200
• 负责人: MATSUI Takashi
• 金额: $ 1.34万
• 依托单位: University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1989
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63560200/
• 关键词: Puffer fish toxin; tetrodotoxin; Kusafugu; Torafugu; toxification; derivatives; クサフグ; アンヒドロテトロドトキシン; フグ毒産生菌; フグの毒化

As a part of the research on the mechanism of toxification in puffer fish, following experiments were conducted.Eggs were obtained from the mature kusafugu and torafugu and fertilized artificially and hatched in aerated aquariums. The larvae were reared with rotifers and copepods for about 20 days in indoor aquariums and then transferred to out door concrete tank and reared with a commercial eel diet for a few years to provide the experimental fish all the time. During this periods, the toxicity was checked at the interval of two month.In various conditions, we examined the accumulation of toxin administered intraperitoneally or subcutaneously. No difference in accumulation was detected in regard to injection methods. The amount of accumulation was directly proportional to the amount of administration and the toxins recovered were 50 and 30 % in kusafugu and torafugu, respectively and were found mainly in the liver of kusafugu and skin of torafugu.The occurrences of TTX derivatives such as anhydTTX, 6-epiTTX, 4-epiTTXt and 11-deoxyTTX were reported. Two of these derivatives were injected into cultured fish and the amount of derivatives remained and the distribution in the fish. Their behavior were almost similar to that of TTX.We examined whether non-toxic puffer fish become toxic by administration of TTX producing bacteria or not. Thirty kusafugu and ten torafugu were fed the diet contained the bacteria for 30 days. The only one torafugu became weakly toxic with toxicities of 0.2 and 1.5 MU/g in the liver and skin, respectively.

作为关于河豚鱼中毒化机制的一部分，进行了实验后进行。幼虫在室内水族馆中饲养大约20天，然后转移到室外混凝土罐中，并用商业鳗鱼饮食饲养几年，以一直提供实验性鱼类。在此期间，在两个月的时间间隔检查毒性。在各种条件下，我们检查了腹膜内或皮下的毒素的积累。关于注射方法未检测到积累的差异。在库萨夫古和托拉夫古（Kusafugu和torafugu）分别与给药量直接成正比，恢复的毒素分别为50％和30％，主要发现在kusafugu和torafugu的肝脏中。报道了6个EPITTX，4-EPITTXT和11-脱氧ttx。这些衍生物中的两个被注入培养的鱼中，并且衍生物的量以及鱼类的分布。它们的行为几乎与TTX相似。我们检查了通过施用TTX产生细菌是否有毒的无毒河豚鱼有毒。喂食饮食30天，饮食中有30个kusafugu和十个Torafugu。唯一的一种Torafugu在肝脏和皮肤中分别具有0.2和1.5 mu/g的毒性毒性弱。

项目成果

TAKASHI MATSUI: "Production of tetrodotoxin by the intestinal bacteria of a puffer fish Takifugu niphobles." Nippon Suisan Gakkaishi. 55. 2199-2203 (1989)

TAKASHI MATSUI：“河豚鱼的肠道细菌产生河豚毒素。”

TAKASHI MATSUI: "toxification of cultured puffer fish by the administration of tetrodotoxin producing bacteria." Nippon Suisan Gakkaishi.

TAKASHI MATSUI：“通过施用河豚毒素产生细菌来使养殖河豚中毒。”

松居隆: 日本水産学会誌.

松井隆：日本水产科学会杂志。

TAKASHI MATSUI: "Toxification of cultured puffer fish by the administration of tetrodotoxin producing bacteria." Nippon Suisan Gakkaishi in press.

松井隆：“通过施用产生河豚毒素的细菌来使养殖河豚中毒。”

TAKASHI MATSUI: "Production of tetrodotoxin by the intestinal bacteria of a puffer fish Takifugu niphobles." Nippon Suisan Gakkaishi 55, 2199-2203, 1989.

TAKASHI MATSUI：“河豚鱼的肠道细菌产生河豚毒素。”