Studies on a mechanism of puffer fish toxin accumulation

河豚毒素积累机制研究

• 批准号: 10660194
• 负责人: MATSUI Takashi
• 金额: $ 2.18万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10660194/
• 关键词: tetrodotoxin; puffer fish toxin; kusafugu; TTX binding protein; toxin accumulation

A tetrodotoxin binding protein has been purified from the plasma of the puffer fish ku safugu, Takifugu niphobles, through DEAE-cellulose treatment, ammonium sulfate fractionation, Sephadex gelfiltrations, and Sephacryl S-200 and Cellulofine A-500 column chromatography. Final purification by HPLC on a TSK G-3000 SL column yielded a protein which showed only a single protein peak . The molecular weight of the protein was estimated to be 116,000 and 96,000 by SDS-PAGE and mass spectrometry, respectively. A blast search on the amino-terminal amino acid sequence of thc purified protein revealed that thc protein had no homology to any other protein on data base.

通过 DEAE 纤维素处理、硫酸铵分级分离、Sephadex 凝胶过滤、Sephacryl S-200 和 Cellulofine A-500 柱层析，从河豚鱼 ku safugu (Takifugu niphobles) 的血浆中纯化出河豚毒素结合蛋白。在 TSK G-3000 SL 柱上通过 HPLC 进行最终纯化，得到仅显示单个蛋白质峰的蛋白质。通过SDS-PAGE和质谱估计该蛋白质的分子量分别为116,000和96,000。对纯化蛋白的氨基末端氨基酸序列进行的爆炸搜索表明，该蛋白与数据库中的任何其他蛋白没有同源性。

项目成果

Takashi Matsui, et al.: "Purification and some properties of a tetrodotoxin binding protein from the blood plasma of kusafugu, takifugu riphobles"

Takashi Matsui 等人：“草河豚、takifugu riphobles 血浆中河豚毒素结合蛋白的纯化和一些特性”

Takashi Matsui, Kunio Yamamori, Kiyoshi Furukawa, and Michiko Kono: "Purification and some properties of a tetrodotoxin binding protein from the blood plasma of kusafugu, Takifugu niphobles"Toxicon. 38(3). 463-468 (2000)

Takashi Matsui、Kunio Yamamori、Kiyoshi Furukawa 和 Michiko Kono：“草河豚血浆中河豚毒素结合蛋白的纯化和一些特性”Toxicon。

Takashi Matsui et al.: "Purification and some properties of a tetrodotoxin binding protein from the blood plasma of kusafugu,Takifugu niphobles"Toxicon. 38・3. 463-468 (2000)

Takashi Matsui 等人：“草河豚血浆中河豚毒素结合蛋白的纯化和一些特性”Toxicon 38・3 (2000)。