Cloning of cDNA encoding for transcription regulatory factor of the laminin B1 gene

编码层粘连蛋白B1基因转录调节因子的cDNA的克隆

• 批准号: 04680176
• 负责人: MATSUI Takashi
• 金额: $ 1.22万
• 依托单位: University of Occupational and Environmental Health
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04680176/
• 关键词: laminin B1; transcriptional induction; regulatory factor; nuclear receptor; ラミニン; プロモーター; 細胞分化

Molecular mechanism by which transcription of the laminin B1 gene is regulated after differentiation of F9 cells by retinoic acid have been analyzed. Using in vitro transcription reconstitution system, we defined a novel promoter structure of the murine laminin B1 gene which is consisted of three elements between -1 and -100. Further, we showed from transient expression analysis that transcription of the laminin B1 gene is activated by AP-1 activity induced after F9 cell differentiation. Interestingly, AP-1 induced activation of transcription was abrogated by a stem cell specific negative repressor which disappeared late after the differentiation. Therefore, it was suggested that the late induction of transcription of the laminin B1 gene regulated by AP-1 in concert withthe negative factor. We currently obtained a cDNA clone encoding a novel orphan unclear receptor, although it is not known at present whether this receptor is involved in regulation of transcription of the laminin B1 gene. Interestingly, the orphan receptor was shown to be expressed strictly in mouse brain and in P19 EC cell which was induced to differentiated into neural cells by RA.This suggested that the orphan receptor might play key role in regulation of genes expressing during development and differentiation of central nervous system.

已经分析了视黄酸在F9细胞分化后调节层粘连蛋白B1基因的分子机制。使用体外转录重建系统，我们定义了鼠层粘连蛋白B1基因的新型启动子结构，该结构由-1和-100之间的三个元素组成。此外，我们从瞬态表达分析中表明，层粘连蛋白B1基因的转录被F9细胞分化后诱导的AP-1活性激活。有趣的是，AP-1诱导的转录激活被干细胞特异性的负抑制剂废除，该干细胞在分化后很晚消失。因此，有人提出，由AP-1调节的层粘连蛋白B1基因的转录与负因子一起调节。目前，我们获得了编码新型孤儿受体的cDNA克隆，尽管目前尚不清楚该受体是否参与了层粘连蛋白B1基因转录的调节。有趣的是，孤儿受体被证明在小鼠脑和p19 EC细胞中被严格表达，该细胞被RA被诱导地分化为神经细胞。这表明孤儿受体可能在调节表达在发育和分化过程中表达的基因的关键作用中枢神经系统。

项目成果

Ryusuke Okano: "Characterization of a novel promoter structure and its transcriptional regulation of the murine laminin B1 gene." Biochemica.Biophysica.Acta. 1132. 49-57 (1992)

Ryusuke Okano：“鼠层粘连蛋白 B1 基因的新型启动子结构及其转录调控的表征。”

Ryusuke Okano: "Characterization of a novel promoter structure and its transcriptional regulation of the murine laminin B1 gene." Biochemica.Biophysica.Acta.1132. 49-57 (1992)

Ryusuke Okano：“鼠层粘连蛋白 B1 基因的新型启动子结构及其转录调控的表征。”

A.Mizokami, H.Saiga, T.Matsui, T.Mita and A.Sugita: "Regulation of androgen receptor by androgen and epidermal growth factor in a human prostaticcance cell line, LNGaP." Endocrinol.Japan. 39. 235-243 (1992)

A.Mizokami、H.Saiga、T.Matsui、T.Mita 和 A.Sugita：“人类前列腺癌细胞系 LNGaP 中雄激素和表皮生长因子对雄激素受体的调节”。

A.Mizokami: "Regulation of androgen receptor by androgen and epidermal growth factor in a human prostatic cancer cell line,LNCaP." Endocrinol.Japan. 39. 235-243 (1992)

A.Mizokami：“人类前列腺癌细胞系 LNCaP 中雄激素和表皮生长因子对雄激素受体的调节。”

R.Okano, T.Mita and T.Matsui: "Characterization of a novel promoter structure and its transcriptional regulation of the murine laminin B1 gene." Biochemica.Biophysica.Acta. 1132. 49-57 (1992)

R.Okano、T.Mita 和 T.Matsui：“鼠层粘连蛋白 B1 基因的新型启动子结构及其转录调控的表征。”