Anti-Aging Effects of Natural Antioxidants on Senescence Accelerated Mouse (SAM)

天然抗氧化剂对加速衰老小鼠 (SAM) 的抗衰老作用

基本信息

批准号：
63580072
负责人：
YAMASHITA Kanae
金额：
$ 1.54万
依托单位：
Sugiyama Jogakuen University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1988
资助国家：
日本
起止时间：
1988 至 1990
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63580072/
关键词：
SAM sesame sesaminol catechin Vitamin E peroxidized lipids anti-aging effects

项目摘要

To investigate the effects of antioxidants in foods on the aging of biological function, senescence accelerated mice (SAM) were used throughout this study as a test animal, in addition to Wistar rats. Sesame seed products, sesaminol isolated from sesame oil and catechin isolated from green tea were used as antioxidants and the following results were obtained.1) By long term feeding with the diets containing high levels of crushed sesame seeds, the peroxidation of lipids in the liver and increases of lipofuscin in the testis were effectively depressed in comparison with the control group without crushed sesame seeds.2) The addition of crushed sesame seeds to the diets suppressed the grading score for evaluation of the degree of senecence of SAM, especially the score for glossiness and coarseness of skin hair.3) Peroxidized lipids in the liver and plasma, and hemolysis and pyruvate kinase activity in the plasma were markedly increased in rats fed a tocopherol deficient diet. The addition of sesame products (crushed sesame seeds and oils) which contained -tocopherol exclusively, lowered these characteristics to the same level as the diets containing -tocopherol. However, the addition of -tocopherol to the tocopherol deficient diet depressed the characteristics less than the diet containing -tocopherol.4) The increase of peroxidized lipids in the liver of SAM fed with tocopherol deicient diets was depressed by the addition of sesaminol (0.1%), a potent antioxidative lignan in sesame oil. The dosage of carbon tetra chloride solution to rats also induced lipid peroxidation in its liver, but it was suppressed to an ordinary level by the addition of sesaminol to the solution.5) On the same experiments using tocopherol deficient diets, a slight depression of lipid peroxidation in liver, kidney and plasma was observed by the addition of catechin and its analogues from green tea to the diets.

为了研究抗氧化剂对食物对生物功能衰老的影响，除Wistar大鼠外，整个研究中都将衰老加速小鼠（SAM）用作测试动物。芝麻籽产物，从芝麻油中分离出来的芝麻和从绿茶中分离出的儿茶素作为抗氧化剂，并获得了以下结果。1）长期饮食中含有高水平碎芝麻种子的饮食，肝脏中脂质过氧化的饮食与对照组相比，睾丸中脂肪霉素的增加有效地抑郁症，而没有压碎的芝麻种子。2）在饮食中添加压碎的芝麻种子，抑制了评估SAM的评估程度的分级评分，尤其是SAM的分数3）肝脏和血浆中的过氧化脂质，以及在喂养饮食中缺乏饮食的大鼠中，血浆中的溶血和丙酮酸激酶活性显着增加。芝麻产品（芝麻碎的种子和油）的添加仅包含 - 生育酚，将这些特征降低到与含有含酚酚的饮食相同的水平。然而，与含含含-2ophopherol的饮食的特征相比，在生育酚缺乏饮食中添加 - 生育酚的特征降低了。4）通过添加芝麻醇（0.1％），SAM喂食的SAM肝脏中的过氧化脂质的增加降低了），芝麻油中有效的抗氧化木质。大鼠碳四氯溶液的剂量也会在其肝脏中诱导脂质过氧化，但通过在溶液中添加芝麻醇，将其抑制至普通水平。通过添加儿茶素及其从绿茶到饮食的类似物，观察到肝，肾脏和血浆中的过氧化。