Development of process for production of anti-aging glycosides from renewable cereal resources

利用可再生谷物资源生产抗衰老糖苷的工艺开发

基本信息

批准号：
10650781
负责人：
MIMURA Akio
金额：
$ 2.24万
依托单位：
Yamanashi University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 2000
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10650781/
关键词：
Cereal resources Wheat bran Sesame oil cake Anti- aging Cell structure proteins Sesaminol glycosides Vimentin Active oxygen species 活性酸素消去活性細胞老化度プライマーセット

项目摘要

(1) Process development to produce antioxidative compounds from by-products from cereal processing industriesMicroorganisms which could produce antioxidative compounds from wheat bran were isolated from soil. The bacterium was determined as Bacillus megaterium YUK-6. The antioxidative compounds thus produced were oily material which are soluble in ethylacetate and easily crystalized under lower temperature in a refrigerator. Chemical structure has not been elucidated.A bacrerium has been isolated from siol that could produce antioxidative glycosides from sesame oil cake which is by-product of sesame oil mill. The bacterium was identified as Bacillus circulans YUS-9. The antioxidative compounds were purified and their chemical structures were elucidated as Sesaminol diglucoside and Sesaninol tri-glucoside. They are considered as the components in sesame seed and liberated by the action of enzymes in the. bacteriun.Thus the process was developped to produce antioxidative compounds from b … More y-products from cereal processing industries.(2) Construction of the evaluation system for activity to repress aging of human cell linesOne characteristic feature of the senescent fibroblasts is flat, enlarged and heterogeneous cell shapes. The present study was aimed to understand the structural basis of the senescent cell morphology. SDS-gel electrophoresis as well as Western blotting demonstrated that there occurred a prominent protein band about 57 kDa in the senescent cells as compared with normal young or immortalized cells growing. rapidly, and the protein was identified with a cytoskeletal protein, vimentin. In fact, senescent fibroblasts contained approximately three-fold more vimentin protein, and four-fold more vimentin mRNA than young embryonic fibroblasts. In the senescent cells, vimentin-cytoskeleton occurred as densely bundled filaments in parallel with the long axis of cell bodies, whereas in young or actively growing cells it showed short and thin vimentin filaments or fur-like irregular networks. It was further demonstrated that senescent cell-shapes could be induced when a vimentin expression construct was transfected in young fibroblasts. These results suggest that senescent fibroblasts overproduce vimentin protein, and the overproduced vimentin fllaments bring about the senescent cell morphology. Less

(1)利用谷物加工副产品生产抗氧化化合物的工艺开发从土壤中分离出能够从麦麸中生产抗氧化化合物的微生物，该细菌被确定为巨大芽孢杆菌YUK-6。由此产生的抗氧化化合物是油状物质。溶于乙酸乙酯，在冰箱低温下容易结晶。化学结构尚未阐明。已从硅醇中分离出细菌。从芝麻油厂副产品芝麻油饼中提取抗氧化苷，经鉴定为Bacillus circulans YUS-9，并纯化其抗氧化化合物，并阐明其化学结构为芝麻酚二葡萄糖苷和芝麻酚三葡萄糖苷。它们被认为是芝麻中的成分，并通过细菌中的酶的作用释放出来。该工艺的开发目的是从谷物加工工业的b… More y-产品中生产抗氧化化合物。(2) 人类细胞系抑制衰老活性评价体系的构建衰老成纤维细胞的一个特征是细胞扁平、增大和异质性本研究旨在了解衰老细胞形态的结构基础，并且蛋白质印迹表明，在衰老细胞中出现了一条约 57 kDa 的显着蛋白质条带。与正常年轻或永生化细胞相比，衰老细胞生长迅速，并且该蛋白质被鉴定为细胞骨架蛋白波形蛋白。事实上，衰老成纤维细胞含有比年轻胚胎多约三倍的波形蛋白和四倍多的波形蛋白mRNA。在衰老细胞中，波形蛋白细胞骨架以与细胞体长轴平行的密集成束的细丝形式出现，而在年轻或活跃生长的细胞中则呈密集成束的细丝形式。显示出短而细的波形蛋白丝或毛皮状不规则网络，进一步证明当波形蛋白表达构建体转染年轻成纤维细胞时，可以诱导衰老细胞形状。这些结果表明衰老成纤维细胞过量产生波形蛋白。纤丝导致细胞形态的衰老较少。