Mechanism of enhancing vitamin E activity by sesame seed or its lignans

芝麻或其木脂素增强维生素E活性的机制

基本信息

批准号：
07680051
负责人：
YAMASHITA Kanae
金额：
$ 1.41万
依托单位：
Sugiyama Jogakuen University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

项目摘要

Mechanisms enhancing vitamin E activity by sesame seed or its lignans were investigated in two experiments.In experiment 1 we examined whether or not the excretion of gamma-tocopherol (Toc) into the bile is suppressed by sesame seed intake, It has been well known that alpha-Toc content is much higher than gamma-toc in the plasma and liver of humans and rats. It has thus been assumed that gamma-Toc, which cannot bind with the alpha-Toc transfer protein is excreted into the bile. However, in a previous experiment, we observed that gamma-Toc content in the plasma and liver was higher in rats fed a sesame seed containing diet than in a gamma-Toc diet with the same gamma-Toc intake. We supposed that sesame seed suppresses gamma-Toc excretion into the bile, so we determined the gamma-Toc contents in the plasma, liver and bile. We found unexpectedly that more gamma-Toc was excreted into the bile when its concentration in the liver was high, and that less gamma-Toc was excreted when its concentration in the liver was low. The very low gamma-Toc concentrations in the liver and bile suggest that gamma-Toc was destroyed very quiclky and the sesame seed may suppress gamma-Toc destruction.In experiment 2 we examined whether or not sesaminol enhanced tocopherol adsorption. Rats were divided into three groups. Group 1 received a diet contatining 20 mg of tocopherol and tocotrienol mixture (Tmix), group 2 received a diet containing 20 mg Tmix with 0.1% sesaminol, and group 3 received either a diet containing 40 mg Tmix or the same diet alternating with 0.2% sesaminol diet without Tmix every other day. Group 1 showed vitamin E deficient status but group 2 and 3 showed significant improvement of vitamin E status. The enhancing effects of vitamin E activities in group 2 and 3 were nearly the same. These results indicated that sesaminol did not cause an enhanced absorption of tocopherol.

在两个实验中研究了芝麻或其木质酶增强维生素E活性的机制。在实验1中，我们检查了γ-生育酚（TOC）是否排泄到胆汁中的排泄被芝麻种子摄入抑制，众所周知，众所周知，在人类和大鼠的血浆和肝脏中，α-TOC含量远高于γ-TOC。因此，已经假定无法与α-TOC转移蛋白结合的伽马 - -TOC被排泄到胆汁中。然而，在先前的实验中，我们观察到，在喂食芝麻籽的大鼠中，血浆和肝脏中的γ-TOC含量高于含有相同γ-TOC摄入量的γ-TOC饮食中的芝麻籽。我们认为芝麻种子会抑制γ-TOC排泄到胆汁中，因此我们确定了血浆，肝脏和胆汁中的γ-TOC含量。我们出乎意料地发现，当肝脏浓度很高时，更多的γ-TOC被排泄到了胆汁中，并且当肝脏浓度较低时，γ-TOC被排出较少。肝脏和胆汁中非常低的γ-TOC浓度表明，γ-TOC被破坏了非常奇怪的，芝麻可能会抑制伽马 - -TOC的破坏。在实验2中，我们检查了芝麻醇是否增强的培养基吸附。大鼠分为三组。第1组接受了饮食抑制20毫克的生育酚和生育三烯醇混合物（TMIX），第2组接受了含有20毫克TMIX的饮食，含有0.1％的芝麻醇，并且第3组接受了含有40 mg TMIX的饮食，或以0.2％的交替饮食每隔一天不带TMIX的芝麻醇饮食。第1组显示维生素E缺乏状态，但第2组和第3组显示出维生素E状态的显着改善。维生素E活性在第2组和第3组中的增强作用几乎相同。这些结果表明，芝麻醇不会引起生育酚的吸收增强。