Protective effects of sesame lignans on senescence accelerate brain in rats and mice

芝麻木酚素对大鼠和小鼠大脑衰老的保护作用

基本信息

批准号：
12680143
负责人：
YAMASHITA Kanae
金额：
$ 2.37万
依托单位：
Sugiyama Jogakuen University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12680143/
关键词：
sesame seed sesame lignans α-tocopherol senescence of brain rats SAMP8 TBARS learning test

项目摘要

Recent studies have shown that oxidative stress is important in the pathogenesis of Alzheimer disease and high doses of vitamin E to Alzheimer disease patients improved the disease state. β-Amyloid (Aβ), which is found abundantly in the brains of Alzheimer disease patients, is cytotoxic to neurons and has a central role in the pathogenesis of the disease. There was a paper to report protective effects of α-tocopherol (α-Toc) on Aβ(1-42)-induced learing and memory deficits in rats. Our previous studies showed that sesame seed and its lignans elevated α-Toc concentrations in brain as well as plasma and liver. In this study, we investigated whether the protective effects of sesame seed on senescence accelerated brain were observed in Aβ infused rats and senescence accelerated mouse (SAM)P8. In Experiment 1, we examined α-Toc concentrations in cerebrum, cerebellum, brain stem and hippocampus in brain and TEARS in brain in rats fed diets containing 500 mg/kg α-Toc alone and 50 mg/kg α-Toc with sesame seed. In Experiment 2, learning and memory in radial maze task were examined in rats infused Aβ and fed diets 50 mg/kg α-Toc alone and 50 mg/kg α-Toc with sesame seed. In Experiment 3, passive avoidance task was examined in SAMP8 mice fed the same diets in Exp.2. We obtained the results that sesame seed group showed higher concentrations of α-Toc in all parts of brain examined compared to 500 mg/kg α-Toc group and lower concentration of TEARS in brain, however we could not observe that sesame seed improved learning and memory on senescence accelerated brain in rats and mice.

最近的研究表明，氧化应激在阿尔茨海默氏病的发病机理中很重要，而对阿尔茨海默氏病患者的高剂量维生素E则改善了疾病状态。 β-淀粉样蛋白（Aβ）在阿尔茨海默氏病患者的大脑中是绝对的，对神经元具有细胞毒性，并且在疾病的发病机理中具有核心作用。有一篇论文报告了α-生育酚（α-TOC）对Aβ（1-42）诱导的学习和记忆的保护作用。我们以前的研究表明，芝麻及其木质豆在大脑以及血浆和肝脏中升高。在这项研究中，我们研究了在Aβ感染的大鼠中观察到芝麻种子对感应加速大脑的受保护作用，而感应加速小鼠（SAM）P8。在实验1中，我们检查了大脑，小脑，脑干和海马中的α-TOC浓度，在喂食含有500 mg/kgα-TOC的大鼠中的脑部和脑部的泪液中，单独使用50 mg/kg/kgα-toc，用芝麻种子喂食。在实验2中，在注入Aβ的大鼠中检查了径向迷宫任务中的学习和记忆，并单独使用50 mg/kgα-TOC和芝麻种子喂养50 mg/kgα-TOC。在实验3中，在Exp.2中喂养相同饮食的SAMP8小鼠中检查了被动回避任务。我们获得的结果是，与500 mg/kgα-TOC组相比，芝麻种子组在大脑的所有部位显示出较高的α-TOC浓度，而大脑中的撕裂浓度较低，但是我们无法观察到芝麻在大鼠和小鼠中芝麻的脑含量改善了脑部的学习和记忆的脑中的脑中的记忆。