Analysis on the optimum site for harvest site in tissue engineering of the human ear shaped cartilate

人耳形软骨组织工程最佳采集位点分析

• 批准号: 17591881
• 负责人: ISOGAI Noritaka
• 金额: $ 2.3万
• 依托单位: Kinki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17591881/
• 关键词: tissue engineering; chondrocyte; mechanical test; メカニカルテスト

In this experiment, we cultured chondrocytes that were harvested chondrocytes from 4 different sites (nasoseptal, articular, costal and auricular cartilage), which were subsequently seeded onto biodegradable poly(LA-CL,50:50) polymer. Chondrocyte-copolymer composites were implanted in vivo, and then analyzed for long term observation that includes gross morphology, histology, and mechanical property. Results on gross morphology revealed that all tissue engineered cartilages retained human ear shape for up to 40 weeks in vivo. Such tissue engineered cartilages as nasoseptal, articular, and auricular origin demonstrated similar mechanical property with the native bovine auricular cartilage. On the contrary, tissue engineered cartilage of costal origin developed mineralization and showed remarkably rigid mechanical property, which is indicative of probable stem cells in cartilage structure. These results lead us to conclude that tissue engineered cartilages of nasoseptal, articular, and auricular origin were appropriate sites as cell sources as they retained three dimensional shape of the human ear with the restoration of original mechanical property, while that of costal origin further needed to regulate the mineralization process.

在该实验中，我们培养了从四个不同位点（鼻孔，关节，肋骨和耳骨软骨）收集的软骨细胞的软骨细胞，随后将其播种到可生物降解的la-cl，50：50）聚合物中。将软骨细胞聚合物复合材料植入体内，然后分析以进行长期观察，其中包括总形态学，组织学和机械性能。关于总形态的结果表明，所有组织工程软骨均在体内保留长达40周的人耳形状。鼻孔，关节和耳原发明的组织工程软骨与天然牛耳软骨表现出相似的机械性能。相反，组织工程的肋骨起源的软骨发展出矿化，并显示出明显的刚性机械性能，这表明软骨结构中可能的干细胞。这些结果使我们得出结论，鼻塞，关节和耳的组织的组织软骨是适当的位点作为细胞源，因为它们保留了人耳的三维形状，并恢复了原始的机械性能，而costal的起源则需要进一步调节矿化过程。

项目成果

Comparison of different chondrocytes for use in tissue engineering of cartilage model structares

不同软骨细胞用于软骨模型结构组织工程的比较

• 发表时间: 2006
• 期刊: Tissue Engineeing Apr;12 (4)
• 作者: Isogai N;Kusuhara H;Ikada Y;Ohtani H;etc
• 通讯作者: etc