Deep observational researches regarding the profound structures, morphogenesis and regeneration of nerves, bloods and muscles existing in the visceral internal organs as well as four limbs, using sui genesis technology of whole-mount staining, and molecul

利用整体染色、分子生物学等独特成因技术，对内脏、四肢等神经、血液、肌肉的深层结构、形态发生和再生进行深入观察研究

基本信息

批准号：
16590139
负责人：
TANAKA Shigenori
金额：
$ 2.24万
依托单位：
Graduate School of Medical Science, Kanazawa University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2005
项目状态：
已结题

项目摘要

Researches of elucidation of form and structure of the sympathetic nerve distributed in the heart, that is, cardiac nerves were furthermore promoted.The cardiac nerves were found to arise numerous in number from very broad areas of the sympathetic trunk, communicating each other very numerously.Furthermore, their patterns or forms of running were much more complicated than those of the cardiac branches of the vagus nerve.As a result, clarification of features of these cardiac nerves are difficult than the cardiac branches of the vagus nerve.Inter alia, human examples applicable for researches are exceedingly large as those of other vertebrate and further more, they communicate each other and their coursing figures are much more complicated than those of the cardiac branches of the vagus nerve. Therefore, the complete clarification of human cardiac nerves are difficult article.In order to resolve this difficulty, we applied new specific technology. Using this technology, we are able to … More grease alcohol by using alcohol, stain nerves with arizarin red S, and to elucidate blood vessels in all experimental examples of humans and other vertebrates.In consequence, the cardiac nerves were found to arise from the sympathetic trunk in a wide area from the cervical until the lowest thoracic level, i.e., ThI0 (and further more ThI2) in human examples.Furthermore, the three-dimensional structures of human cadavers have been clarified fundamentally not only from the human anatomical point of view, but also comprehensively from the vertebrate comparative anatomy and embryology.We have been made presentations of these results in academic conferences (the 8^<th> Meeting of Japanese Research Society of Clinical Anatomy and the 16^<th> Meeting of Japanese Research Society of Periphera Nerve).Therewithal, the contribution manuscripts of the international science magazine concerning the form of the cardiac nerves and branches are being to retouched and corrected.The clarification of morphology and morphogenesis of the cardiac nerves are performed by using methods for whole embryo immunity staining in muskrats which are nearer human's form than the mouse and the rat for the form formation (doctor's course student's submitting thesis).These results of this research were presented in the 110th in Japanese Anatomy Association. Contribution to the international science magazine is also being prepared. The form of generation of the muskrat liver, clinical medicine meaning (Endoclinology 2004 : Anat.Enbryol. 2004) has been clarified. Furthermore, figures of the nerves innervating the levator palate muscle and mandibular nerve were elucidated (Clin Anat 2004 ; Surg Radiol Anat 2004).At the moment, we make exertions to advancing researches in order to analyze the intimate structures as well as morphogenesis of arteries, veins and nerves coursing in the falciform and teres hepatic ligaments and to clarify the morphological as well as functional relationships-of lymphatic vessel around the xiphoid process with the live in the human cadavers and also in muskrats. These structures and root meanings of these structures are being clarified from the embryological and clinical viewpoints, building up co-operation with clinical researchers. We are also making collaborative researches with doctors in order to open up new method of measurement, diagnosis and treatment of abnormal bones, and also the molecular and histochemical researches of genesis of head and neck muscles. Less

在心脏中分布着交感神经的形式和结构的研究的研究，即进一步促进心脏神经。发现心脏神经在相互交流的非常广泛的彼此之间的广泛沟通，彼此之间的交流非常多。furthermore，奔跑的模式或形式更为复杂，这些范围的范围更为复杂。与迷走神经的心脏分支相比，适用于研究的人类例子明显大于其他脊椎动物的示例，而且更重要的是，它们相互交流，而他们的求婚数字比迷走神经的心脏分支的求婚数字要复杂得多。因此，对人类心脏神经的完全澄清是困难的文章。为了解决这一困难，我们采用了新的特定技术。使用这项技术，我们能够……通过使用酒精，带有亚利桑那州红S的染色神经以及在所有实验人类和其他脊椎动物的实验示例中阐明血管的更多油脂，因此，发现心脏神经是由从颈部的较广泛的胸腔造成的，直到最高的胸腔，thier thiore，thiior，以及更多的thiires thiiore，thi的典范，并进一步构成了更多的thi thi。 the three-dimensional structures of human cadavers have been clarified fundamentally not only from the human anatomical point of view, but also comprehensively from the vertebrate comparative anatomy and embryology.We have been made presentations of these results in academic conferences (the 8^<th> Meeting of Japanese Research Society of Clinical Anatomy and the 16^<th> Meeting of Japanese Research Society of Periphera Nerve).Therewithal, the contribution manuscripts of the international science magazine Concerning the form of the cardiac nerves and branches are being to retouched and corrected.The clarification of morphology and morphogenesis of the cardiac nerves are performed by using methods for whole embryo immunity staining in muskrats which are nearer human's form than the mouse and the rat for the form formation (doctor's course student's submitting thesis).The results of this research were presented in the 110th在日本解剖协会中。还准备对国际科学杂志的贡献。肌肉肝脏的产生形式，临床医学意义（Endoclinology 2004：Anat.enbryol。2004）已阐明。此外，阐明了神经支配悬浮液的神经的数字（Clin Anat 2004; Clin Anat 2004; Surg Radiol Anat，2004年）。在那一刻，我们为进步的研究做出了努力，以分析亲密结构以及动脉，静脉和神经的形态，并在素描和神经的形态上进行彻底和神经的形态，并在脑海中和神经形成，并在他的脑海中和神经形成了。 Xiphoid过程周围的淋巴管和功能关系，与人类尸体以及麝香鼠的生活。这些结构的这些结构和根本含义是从胚胎学和临床观点阐明的，与临床研究人员建立了合作。我们还与医生进行了协作研究，以打开新的测量方法，诊断和治疗异常骨骼的方法，以及头颈肌肉起源的分子和组织化学研究。较少的