Three-dimensional analysis of the morphogenesis of nerve branches and vessels distributed to the heart and bronchial organs of embryos, using whole-mount immunohistochemical staining and moleculohistoochemical methods.

使用整体免疫组织化学染色和分子组织化学方法对分布到胚胎心脏和支气管器官的神经分支和血管的形态发生进行三维分析。

基本信息

批准号：
12670010
负责人：
TANAKA Shigenori
金额：
$ 2.18万
依托单位：
Kanazawa University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12670010/
关键词：
development peripheral nerve heart humans house shrew chick rat immunolhistochemistry

项目摘要

In year of 2000, the branches of the synpathetic nerve which reach the heart through the venous porta was newly discovered in the hearts of house shrew ( Smcus miriws). These nerve branches were observed to originate from the sympathetic trunk, and to course the azygos vein which course in parallel with this sympathetic trunk. Thereafter, these sympathetic (cardiac) nerve branches take a course along the brachiocephalic and superior caval vein, - entering the venous porta of the heart. These nerve branches are not observable through the common anatomical methods, and have not described in scientific papers. In our study, the existence of these heart nerve branch of this sympathetic nerve were clarified-by the method of what is called "whole-mount immunohistochemical staining method".In 2001, on the base of the data mentioned above, we researched concerning the existence of the cardiac nerve of the sympathetic nerve trunk origin in human beings. The observation area of humans is greatly … More wide. The cardiac nerve branches of the sympathetic nerve trunk origin are thinner than the cardiac branches of the vagus nerve origin, and their form is more complex than that of the latter. Therefore, it is difficult in a usual and macroscopic anatomy methods to investigate the concerned branches completely, and to name them. In two (2001 and 2002) years of our research details of the cardiac nerve which arise from the cervix part and the chest sympathetic nerve trunk are sketched in the A2 papers. We have clarified the cardiac nerve branches with the embryology form concept of the arterial and venous portae of the heart.It turned out all nerves distributed in human heart can be named according.to the same terms which we have applied to the cardiac nerve branches of the house shrews (Tanaka et al, 1988).From now on, in our laboratory, embryological researches on the heart nerve branch are scheduled to be proceeded furthermore, while giving a deep mind to the data of humans.Details of the formation of raf s knee joint were also elucidated in the study on the nerve developmental formation of the knee joint. By immunohistochemical staining and biochemical research, it was clarified that the knee joint is not formed with the apoptosis of the cell as hitherto described, but with the ordinal cell development. Less

2000年，在家鼩（Smcus miriws）的心脏中新发现了通过静脉门到达心脏的交感神经分支，这些神经分支被观察到起源于交感干，并走行于奇点。此后，这些交感（贲门）神经分支沿着头臂静脉和上腔静脉走行，进入静脉门。这些神经分支无法通过常见的解剖方法观察到，并且在科学论文中也没有描述过，在我们的研究中，通过所谓的“整体”方法阐明了这种交感神经的这些神经分支的存在。 2001年，我们在上述数据的基础上，研究了人类中是否存在交感神经干起源的心神经。人类的观察范围非常广泛。神经交感神经干起源的分支比迷走神经起源的贲门分支细，而且形态也比后者复杂，因此，用通常的宏观解剖方法很难对相关分支进行彻底的研究。在我们的两年（2001年和2002年）研究中，我们在A2论文中概述了源自子宫颈部分和胸部交感神经干的心神经分支。胚胎学形成了心脏动脉和静脉门的概念。事实证明，分布在人类心脏中的所有神经都可以根据我们应用于家鼩的心脏神经分支的相同术语来命名（Tanaka et al, 1988 ）。今后，在我们的实验室，计划进一步进行心脏神经分支的胚胎学研究，同时深入思考人类的数据。raf膝关节的形成细节是在对膝关节神经发育形成的研究中也阐明了，通过免疫组织化学染色和生化研究，阐明膝关节并不是像迄今为止所描述的那样随着细胞的凋亡而形成，而是随着细胞的有序发育而形成。