The effects of hormones on trafficking in the endolymphatic sac

激素对内淋巴囊运输的影响

• 批准号: 14571622
• 负责人: KAKIGI Akinobu
• 金额: $ 2.18万
• 依托单位: Kochi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571622/
• 关键词: endocytosis; endolymphatic sac; HRP; microperoxidase; cationized ferritin

To investigate the properties of endocytosis of the epithelial cells in the intermediate portion of the endolymphatic sac (ES), we infused cationized ferritin (CF), microperoxidase (MPO), and horseradish peroxidase (HRP), as endocytosis tracers, into the endolymphatic space of the ES. Thirty minutes after infusion, the tissues were fixed and the distributions of HRP, MOP and CF were observed by transmission electron microscopy. The results at 30 minutes after tracer infusion were as follows. Concerning CF, endocytosis showed different activity depending on the area of the ES. In one area, CF was hardly observed within the epithelial cells, while in another area, it was observed within the epithelial cells. The CF-loaded vesicles were mainly coated vesicles. These results suggested that infusion of the artificial endolymphatic sac endolymph with CF may not stimulate the whole signal system of the ES. Concerning HRP, it bound to the apical membrane and was observed in the vesicles and the sorting endosomes. The diameters of these vesicles were of two types : one had a smaller diameter (less than 200nm), while the other had a larger diameter (over 200nm) and originated from macropinocytosis. Concerning MPO, it was much less observed in the vesicles than HRP. We discuss the different activities of endocytosis among these tracers, and suggest that a large molecular weight substance, i.e.HRP, might stimulate the fluid-phase endocytosis and/or adsorptive pinocytosis.

为了研究内淋巴囊（ES）中间部分上皮细胞的内吞作用特性，我们将阳离子化铁蛋白（CF）、微过氧化物酶（MPO）和辣根过氧化物酶（HRP）作为内吞作用示踪剂注入内淋巴空间ES 的。输注30分钟后，固定组织，透射电镜观察HRP、MOP和CF的分布。示踪剂输注后30分钟的结果如下。对于 CF，根据 ES 的区域，内吞作用表现出不同的活性。在一个区域中，在上皮细胞内几乎观察不到CF，而在另一区域中，在上皮细胞内观察到CF。负载CF的囊泡主要是包被的囊泡。这些结果表明，人工内淋巴囊内淋巴输注CF可能不会刺激ES的整个信号系统。关于 HRP，它与顶膜结合，并在囊泡和分选内体中观察到。这些囊泡的直径有两种类型：一种直径较小（小于200nm），另一种直径较大（超过200nm）且源自巨胞饮作用。关于 MPO，在囊泡中观察到的 MPO 比 HRP 少得多。我们讨论了这些示踪剂中内吞作用的不同活性，并表明大分子量物质，即 HRP，可能会刺激液相内吞作用和/或吸附胞饮作用。

项目成果

Endocytosis in the epithelial cells of the endolymphatic sac.

内淋巴囊上皮细胞的内吞作用。

• 发表时间: 2004
• 期刊: Acta Histochem Cytochem 37
• 作者: Kakigi A;Okada T;Takeda T;Takeuchi S;Sawada S;Higashiyama K;Azuma H;Yamakawa K;Seguchi H
• 通讯作者: Seguchi H