HORMONAL REGULATION OF INNER EAR FLUID IN THE INTERMEDIATE CELLS OF THE STRIA VASCULARIS

血管纹中间细胞内耳液的激素调节

基本信息

批准号：
09671750
负责人：
KAKIGI Akinobu
金额：
$ 1.98万
依托单位：
KOCHI MEDICAL SCHOOL
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

项目摘要

Clinically, we have reported that plasma antidiuretic hormone (ADH) increased in patients with endolymphatic hydrops (e. g. Meniere's disease). In this project, guinea pigs were administrated ADH in order to reveal if ADH has any relations to produce endolymphatic hydrops. Administration of ADH produced endolymphatic hydrops in guinea pigs. Next, an antagonist of ADH was administered in guinea pig with endolymphatic hvdrops, which was made by obstruction of the endolymphatic sac andlor duct. The antagonist of ADH made folding of the Reissner membrane, which means the endolymphatic hydrops, was reduced. These results suggest that production of the endolymph may be regulated by hormone i.e. ADH.We suspect the effecting site of this ADH may he the intermediate cell in the stria vascularis because the intermediate cells surround the capillary in the stria vascularis. For the time being, we could not make isolate this intermediate cell. We are revising the intermediate cell isolation techni … More que. We will continue this project which is an investigation of effects of ADH on the isolated intermediate cells.Concerning to other related investigations, we researched "Effect of artificial endolymph injection into the cochlear duct on the endocochlear potential" and "Elongated pericyte-like cells connect discrete capillaries in the cochlear stria vascularis of gerbils and rats". In "Effect of artificial endolymph injection into the cochlear duct on the endocochlear potential", artificial endolymph was injected into the endolymphatic space. The injection of artificial endolymph produced transient increase of endolymphatic potential (EP) amplitude and decrease of negative EP amplitude during anoxia. In "Elongated pericyte-like cells connect discrete capillaries in the cochlear stria vascularis of gerbils and rats", bridging structures between discrete capillaries in the stria vascularis were studied morphologically in gerbils and rats. Strial thin sections for transmission electron microscopy revealed that elongated cells surrounded by the basal lamina provided the structural basis for the bridging structure. Visualization of the basal lamina by immunofluorescent methods revealed that capillaries were often bent at the site of attachment of the bridging cell. Less

临床上，我们报道了内淋巴积水（例如梅尼埃病）患者血浆抗利尿激素（ADH）升高。在本项目中，给豚鼠注射ADH，以揭示ADH是否与产生内淋巴积水有关。在豚鼠中产生内淋巴水肿接下来，将 ADH 拮抗剂与内淋巴水肿一起给予豚鼠，这是由内淋巴囊和/或导管阻塞引起的。ADH 的拮抗剂使 Reissner 膜折叠，这意味着内淋巴水肿减少。这些结果表明内淋巴的产生可能受到激素（即 ADH）的调节。该ADH的作用部位可能是血管纹中的中间细胞，因为血管纹中中间细胞围绕着毛细血管，目前我们还无法确定。我们正在修改中间细胞分离技术...更多 que 我们将继续这个项目，该项目是研究 ADH 对分离的中间细胞的影响。关于其他相关研究，我们研究了“人工内淋巴注射的效果”。进入耳蜗管的内耳蜗电位”和“细长的周细胞样细胞连接沙鼠和大鼠耳蜗血管纹中离散的毛细血管”。 “人工内淋巴注射到耳蜗管对耳蜗内电位的影响”，人工内淋巴注射到内淋巴空间中，在缺氧期间产生内淋巴电位（EP）振幅的短暂增加和负EP振幅的降低。 “细长的周细胞样细胞连接沙鼠和大鼠耳蜗血管纹中离散的毛细血管”，在离散的结构之间桥接结构对沙鼠和大鼠血管纹毛细血管的形态学研究显示，基底层周围的细长细胞为桥接结构提供了结构基础。通常在桥接细胞的附着处弯曲较少。