The creation of a digital shareable archive of high-resolution images of the human temporal bone tissue

创建人类颞骨组织高分辨率图像的数字共享档案

基本信息

批准号：
10451412
负责人：
Akira Ishiyama
金额：
$ 22.46万
依托单位：
UNIVERSITY OF CALIFORNIA LOS ANGELES
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-12-01 至 2022-11-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10451412
关键词：
Animals Archives Auditory Automobile Driving Autopsy Axon Basic Science Bone Tissue Celloidin Charge Chicago Clinical Clinical Research Cochlea Collaborations Collection Communities Databases Dendrites Development Digital Libraries Ear Endolymphatic duct Equipment Eustachian Tube External auditory canal Funding Goals Grant Hematoxylin and Eosin Staining Method Histologic Histology Histopathology Human Image Imaging Techniques Improve Access Laboratories Labyrinth Light Morphology National Institute on Deafness and Other Communication Disorders Otolaryngology Paper Pathology Pharmacotherapy Records Registries Research Research Personnel Resolution Science Scientist Slide Specialist Specimen Stains Structure System Techniques Temporal bone structure Time Tissues Training Universities Validation digital digital imaging endolymphatic sac high resolution imaging human imaging imager imaging system middle ear parent grant preservation tissue preparation tool

项目摘要

ABSTRACT In the present supplement to the U24 grant 1U24DC015910 for the NIDCD National Temporal Bone Laboratory at UCLA, we aim to make available human inner ear histopathological images for educational and scientific purposes. The specific aim of this supplement to our U24 is the creation of a high-resolution digital archive of the NIDCD National Temporal Bone specimens. Temporal bone H&E digitized images will be shared to the NIDCD Registry and Database. The NIDCD National Temporal Bone Laboratory at UCLA houses one of the largest collections of archival temporal bones (approximately 1200 pairs) in USA. Under the U24 Human Temporal Bone grant, the Laboratory has actively pursued the preservation and acquisition of temporal bones and has collaborated with many basic scientists and clinician scientists. We have met the specific aims of the U24 parent grant: 1) to improve access for the auditory and vestibular research community to human inner ear tissues 2) Optimization of tissue preparation techniques 3) the development of imaging techniques to visualize human middle and inner ear and 4) the training of residents and scientists in the study of the human temporal bone histopathology. The UCLA archival human temporal bone specimens are unique since many of our specimens were obtained with minimal postmortem time allowing for superb morphological preservation. In order to disseminate the human temporal bone histopathology as an educational tool, and as a means of collaboration with scientists, we submit the present request for supplement to the NIDCD National Temporal Bone Laboratory at UCLA for specialized equipment to allow for the creation of high-resolution digital library. The use of celloidin-embedded human temporal bones has been instrumental in driving basic animal inner ear research into the understanding of human ear pathology and has a high potential for the development of effective drug therapies for restoring auditory and vestibular function. The materials generated in this proposal aids research scientists who are conducting animal studies in auditory and vestibular research by providing validation of their findings in the human. The imaging system requested in the supplement will allow to create a high-resolution digital image of hematoxylin and eosin sections of all the temporal bones available in our laboratory. These digital images will be uploaded to the National Temporal Bone registry and database.

抽象的在目前 U24 补助金 1U24DC015910 的补充中，用于 NIDCD 国家颞骨加州大学洛杉矶分校实验室，我们的目标是提供人类内耳组织病理学图像，用于教育和科学目的。我们的 U24 补充的具体目标是创建高分辨率数字 NIDCD 国家颞骨标本档案。颞骨 H&E 数字化图像将被共享到 NIDCD 注册和数据库。加州大学洛杉矶分校 (UCLA) 的 NIDCD 国家颞骨实验室拥有以下实验室之一：美国最大的颞骨档案馆藏（约 1200 对）。 U24人类下颞骨资助，实验室积极致力于颞骨的保存和获取并与许多基础科学家和临床科学家合作。我们已经实现了具体目标 U24 家长资助：1) 改善听觉和前庭研究界对人类内耳的了解组织 2) 组织制备技术的优化 3) 可视化成像技术的发展人类中耳和内耳，4) 对住院医生和科学家进行人类颞叶研究的培训骨组织病理学。加州大学洛杉矶分校档案人类颞骨标本是独一无二的，因为我们的许多在最短的尸检时间内获得标本，从而实现了极好的形态保存。在为了传播人类颞骨组织病理学作为一种教育工具，并作为一种手段与科学家合作，我们向 NIDCD 国家时间数据库提交了目前的补充请求加州大学洛杉矶分校的骨骼实验室提供专用设备，可用于创建高分辨率数字图书馆。使用嵌入赛璐珞的人类颞骨有助于驱动基本的动物内耳研究了解人耳病理学，具有很高的发展潜力恢复听觉和前庭功能的有效药物疗法。本提案中生成的材料通过提供帮助进行听觉和前庭研究动物研究的科学家在人体中验证他们的发现。补充中要求的成像系统将允许创建一个我们提供的所有颞骨的苏木精和伊红切片的高分辨率数字图像实验室。这些数字图像将上传到国家颞骨登记处和数据库。