Toxification of puffer and tetrodotoxin-associated proteins in liver

河鲀和河豚毒素相关蛋白在肝脏中的毒性

• 批准号: 14560170
• 负责人: NAGASHIMA Yuji
• 金额: $ 1.92万
• 依托单位: Tokyo University of Marine Science and Technology (Tokyo University of Fisheries)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14560170/
• 关键词: Puffer; Toxification; Tetrodotoxin; Liver tissue slices; In vitro accumulation; Tetrodotoxin-associated proteins; Transporter; Inhibitors; 麻痺性貝毒

The liver tissue slices of pufferfish accumulate in vitro tetrodotoxin (TTX), when incubated with minimum essential medium containing TTX. In the case of Takifugu rubripes liver slices incubated at a concentration of 25μg TTT/ml, TTX of 3.9μg/g was first detected at 2h and increased to 15.0μg/g at 48h. The TTX content accumulated was first detected at 2h and increased to 15.0μg/g at 48h. The TTX content accumulated was not decreased, even when the slices were further incubated without TTX for additional 48h. On the contrary, in the cases of the liver slices from parrot-bass Oplegnathus fasciatus, green ling Hexagrammos otakii and filefish Thamnaconus modestus incubated at a concentration of 25μg TTX/ml, TTX of 3-4μg/g was detected even at 0.5h. However, no significant change in TTX contents was recognized during the incubation for 48h. Further incubation of the filefish liver slices without TTX for additional 48h did not decrease the TTX content. It is unlikely that the liver slices of slices of filefish as well as pufferfish rapidly excrete TTX.Effect of inhibitors of transport system on in vitro accumulation of TTX in T.rubripes liver tissue slices was examined. Addition of p-aminohippuric acid and carnitine to the culture media significantly decreased in TTX content accumulated in the puffer liver tissue slices while that of tetraethylammonium did not, suggesting the involvement of an organic ion transporter in TTX accumulation in puffer liver.

当与含有 TTX 的最低必需培养基一起培养时，河豚的肝组织切片会在体外积累河豚毒素 (TTX)。在以 25μg TTT/ml 浓度培养的红鳍东方鲀肝切片中，首先检测到 3.9μg/g 的 TTX。 2h后增加至15.0μg/g，48h时TTX含量首次检测到，并增加至15.0μg/g。 15.0μg/g，48小时，即使在没有TTX的情况下进一步孵育切片48小时，积累的TTX含量也没有减少。和锉鱼 Thamnaconusmodeus 以 25μg TTX/ml、TTX 3-4μg/g 浓度孵育即使在 0.5 小时时也检测到 TTX 含量，但在 48 小时的培养过程中，进一步培养不含 TTX 的锉鱼肝脏切片并不会降低切片中的 TTX 含量。锉鱼和河豚快速分泌TTX。研究了转运系统抑制剂对红鳍鱼肝组织切片中TTX体外积累的影响。在培养基中添加对氨基马尿酸和肉碱可显着降低河鲀肝组织切片中积累的TTX含量，而四乙铵则没有，这表明有机离子转运蛋白参与了河豚肝组织切片中TTX的积累。

项目成果

Yuji Ngashima: "In vitro accumulation of tetrodotoxin in pufferfish liver tissue slices"Toxicon. 41・5. 569-574 (2003)

Yuji Ngashima：“河豚毒素在河豚肝组织切片中的体外积累”Toxicon 41・574（2003）。

Yuji Nagashima: "In vitro accumulation of tetrodotoxin in puffer fish liver tissue slices"Toxicon. 41. 569-574 (2003)

Yuji Nagashima：“河豚毒素在河豚鱼肝组织切片中的体外积累”Toxicon。

Yuji Nagashima: "In vitro accumulation of tetradotoxin in pufferdish liver tissue slices"Toxicon. 41・5. 569-574 (2003)

Yuji Nagashima：“河豚毒素在河豚肝组织切片中的体外积累”Toxicon 41・5（2003）。

Yuji NAGASHIMA: "In vitro accumulation of tetrodotoxin in pufferfish liver tissue slices."Toxicon. 41. 569-574 (2003)

Yuji NAGASHIMA：“河豚毒素在河豚肝组织切片中的体外积累。”Toxicon。