Studies on the Production of Tetrodotoxin and the Toxification of Pufferfish.

河豚毒素的产生及河豚毒性的研究。

• 批准号: 61304025
• 负责人: SHIMIZU Chiaki
• 金额: $ 10.5万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Co-operative Research (A)
• 财政年份: 1986
• 资助国家: 日本
• 起止时间: 1986 至 1987
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-61304025/
• 关键词: Tetrodotoxin(TTX); Tetrodotoxin prodotoxin productive bacteria; Tetrodotoxin derivartive; Toxification of Pufferfish; Newt; ニホンイモリ; TTX(テトロドトキシン; TTXに対する抵抗性

1. In this research, the tetrodotoxin (TTX) productive bacteria isolated from Jania sp., Octopus maculosus, Zosimus aeneus, Carcinoscorpius rotundicauda and pufferfishes are 19 strains of Vibrio naceae including 6 strains of V. alginolyticus sp., 8 strains of Pseudomonas alteromonas sp. including A. alga sp. nov. and A. tetrodonas sp. nov. and Sewanella putrefaciens sp., but the TTX productive capacity of these bacteria is decrease extremely when cultured in a culture medium or preserved for a long time. Therefore, the explanation of TTX bio-synthesis or toxification of pufferfish with these bacteria are task of extreme difficulty.2. New detective method of TTX in urine or plasma was discovered.3. TTX analogs identified from pufferfish are 6-epi TTX and 11-deoxy TTX, and from newt are 6-epi TTX, 11-deoxy TTX, anhydro-6-epi TTX, 11-deoxy-4-epi TTX and anhydro-11-deoxy TTX.4. When njected TTX and anhydro TTX mixture (1 : 4) to abdominal cavity of pufferfishes, the reciprocal conversion between both are very few. Accordingly, it is out of the bonds of possibility that anhydro TTX is the source of TTX in pufferfish.5. Nonpoisonous pufferfishes bred with poisonous pufferfihes in the sea water contained high percentage of antibiotics are toxify, so it is probable that a TTX productive bacteria is not the source of toxification in research.6. Toxicity of japanese newt is more than toxicity of poisonous pufferfish's liver.7. TTX is not need to spawning and growth in poisonous pufferfish.

1。在这项研究中，从Jania sp。，章鱼Maculosus，Zosimus aeneus，Carcinoscorpius rotundicauda和Pufferfishes分离出的四毒素（TTX）生产性细菌是19种颤动的纤维naceae菌株，其中包括6个Alginolyticus spsspssspseomass spseomass spseomonas spseomonas spse spse。包括A. alga sp。十一月。和A. Tetrodonas sp。十一月。和Sewanella putrefaciens sp。，但是在培养基中培养或长期保存时，这些细菌的TTX生产能力极高。因此，用这些细菌对TTX生物合成或助长鱼的毒化的解释是极端难度的任务。2。发现了尿液或血浆中TTX的新侦探方法3。从pufferfish鉴定的TTX类似物是6-EPI TTX和11-脱氧TTX，NEWT为6-EPI TTX，11-脱氧TTX，anydro-6-Epi TTX，11-Deoxy-4-Epi TTX和Anhydro-11-Deoxy Ttx.4。当Neject的TTX和Anhydro TTX混合物（1：4）与河豚的腹腔时，两者之间的相互转化很少。因此，Anhydro TTX是Pufferfish中TTX的来源5。在海水中繁殖有有毒的粉末的非繁殖粉鱼含有高百分比的抗生素是毒性的，因此TTX生产性细菌很可能不是研究中的毒性来源。6。日本纽特的毒性不仅仅是有毒puffer鱼的肝脏的毒性7。 TTX不需要在有毒的河豚中产卵和生长。

项目成果

松居隆,山森邦夫,佐藤英雄,清水千秋: 日本水産学会誌. 53. (1987)

Takashi Matsui、Kunio Yamamori、Hideo Sato、Chiaki Shimizu：日本水产科学学会杂志 53。（1987 年）

Takeshi, Yasumoto: "Recent research for the teterodotoxin." Seikagaku. 59. 1321-1325 (1987)

Takeshi，Yasumoto：“河豚毒素的最新研究。”

Tskashi, Matsui: "Studies on the accumulation of the tetrodotoxin and anhydro tetrodotoxin in the pufferfishes." Nippon Suisan Gakkaishi,.

Tskashi, Matsui：“河豚毒素和脱水河豚毒素在河豚体内积累的研究。”

Yasumoto, Takeshi: J. Am. Chem. Soc.,.

安本武：J. Am。

Nari, Yotsu: "Production of tetrodotoxin and its derivatives by Pseudomonas sp. isolated from the skin of a pufferfish." Toxicon. 25. 225-228 (1987)

Nari, Yotsu：“从河豚皮肤中分离出的假单胞菌产生河豚毒素及其衍生物。”