Origin of tetrodotoxin and toxification mechanism in juvenile puffer fishes

河鲀幼鱼河豚毒素的来源及中毒机制

基本信息

批准号：
14560159
负责人：
YAMAMORI Kunio
金额：
$ 1.92万
依托单位：
Kitasato University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2003
项目状态：
已结题

项目摘要

The toxification of puffer by tetrodotoxin is an important subject. The facts that the toxicity in natural puffer varied regionally and individually, and that the cultured puffer was innocuous may suggest that the toxification was caused by the food chain mechanism. In Okkirai bay, Iwate prefecture, highly toxic juvenile puffer fish appear in summer and autumn. Then, to have actual proof of the food chain mechanism we conducted rearing experiments in Okkirai Bay. Non-toxic cultured juvenile kusafugu Takifugu niphobles were confined in a plastic fish cage (80x56x37cm) submerged in seawater and fed natural living organisms intruding into the cage from outside through narrow gap. The rearing experiments were conducted for two to four weeks at two spots in Okkirai Bay. (Onisawa fishing port and Sodenosawa) and repeated during summer and autumn for three years. After each rearing experiment, the toxicity of specimens used were assayed using mouse to monitor whether toxification had occurred … More or not. At Sodenosawa among total 14 experiments conducted none showed toxification occurred. On the other hand, at Onisawa among 14 experiments 5 experiments conducted from August to October showed toxification occurred. Above result suggest that non-toxic cultured juvenile kusafugu become toxic when reared in a cage submerged in seawater in certain times at certain spots.In the experiments described above, toxification of cultured juvenile puffer was supposed to be occurred by ingesting natural living organisms, but the causative organisms were not identified. Then, in the next experiment, we tried to narrow the range of the causative organisms. Fish rearing tanks containing non-toxic cultured juvenile kusafugu were set up near Onisawa fishing port. Seawater of the port was pumped up and filtered by 2, 0.3 and 0.1 mm mesh filters respectedly and sequentially. Filtered and condensed microorganisms with 0.3 or 0.1 mm mesh filters were provided to the rearing tanks and fed to the kusafugu. In the experiment conducted from August to October showed toxication occurred. This suggests that the causative organisms are very small. Less

四毒素对河豚的毒素是一个重要的主题。在14个实验中，使用小鼠监测毒化是否发生在14个实验中。在某些时期，关于自然活的生物发生的实验，但在下一个因果鱼类养殖罐中，未识别出病原体的生物。端口，由2.1毫米的MEM网格过滤，并依次使用0.3或0.1 mm MM网状滤镜。显示毒性发生。

项目成果

期刊论文数量（18）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Kunio Yamamori, Michiko Kono, Kiyoshi Furukawa, Takashi Matsui: "The toxification of juvenile cultured kusafugu Takifugu niphobles by oral administration of Crystalline tetrodotoxin (in Japanese)"Shokuhin Eiseigaku Zasshi. 45-2. 73-75 (2004)

Kunio Yamamori、Michiko Kono、Kiyoshi Furukawa、Takashi Matsui：“口服结晶河豚毒素对幼体养殖草东方鲀的毒性（日语）”Shokuhin Eiseigaku Zasshi。

DOI：
发表时间：
期刊：
影响因子：
0
作者：
通讯作者：

山森邦夫: "フグにおけるフグ毒の存在形態"日本水産学会誌. 68巻6号. 923-924 (2002)

Kunio Yamamori：“河豚毒素在河豚鱼中的存在形式”，日本水产学会杂志，第 68 卷，第 6 期，923-924（2002 年）。

DOI：
发表时间：
期刊：
影响因子：
0
作者：
通讯作者：

Kunio Yamamori 他8名(Kazuhito Nakashima): "Occurrence of saxitoxins as a major toxin in the ovary of a marine puffer Arothron firmamentum"Toxicon. 43巻2号. 207-212 (2004)

Kunio Yamamori 等 8 人（Kazuhito Nakashima）：“石房蛤毒素作为海洋河豚 Arothron fimamentum 卵巢中的主要毒素的出现”Toxicon，第 43 卷，第 2 期，207-212（2004 年）。

DOI：
发表时间：
期刊：
影响因子：
0
作者：
通讯作者：

Kunio Yamamori 他計8名(Kazuhito Nakashima): "Occurrence of saxitoxins as a maior toxin in the ovary of a marine puffer Arothron firmamentum"Toxicon. (印刷中). (2004)

Kunio Yamamori 和其他 8 人（Kazuhito Nakashima）：“石房蛤毒素作为海洋河豚 Arothron fimamentum 卵巢中的主要毒素的出现”Toxicon（2004 年出版）。

DOI：
发表时间：
期刊：
影响因子：
0
作者：
通讯作者：

Kazuhito Nakashima, Osamu Arakawa, Shigeto Taniyama, Mamoru Nonaka, Tomohiro Takatani, Kunio Yamamori, Yuichi Fuchi, Tamao Noguchi: "Occurrence of saxitoxin as a major toxin in the ovary of a marine puffer Arothron firmamentum"Toxicon. 43-2. 207-212 (2004

Kazuhito Nakashima、Osamu Arakawa、Shigeto Taniyama、Mamoru Nonaka、Tomohiro Takatani、Kunio Yamamori、Yuichi Fuchi、Tamao Noguchi：“石房蛤毒素作为海洋河豚 Arothron fimamentum 卵巢中主要毒素的存在”Toxicon。