Mechanisms responsible for the attenuating effects of estrogen on cardiovascular responses to psychological stress in the central nervous system and the peripheral vessels

雌激素减弱中枢神经系统和外周血管对心理应激的心血管反应作用的机制

基本信息

批准号：
17590202
负责人：
MORIMOTO Keiko
金额：
$ 2.3万
依托单位：
Nara Women's University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

项目摘要

(1) Sex difference in norepinephrine surge in response to psychological stress through nitric oxide in ratsA mild psychological stress induced by a cage-switch evoked a norepinephrine surge in the male, but not in the female rats. There was a sex difference in the norepinephrine surge in response to psychological stress through nitric oxide (NO), in association with pressor response.(2) Effects of estrogen on plasma catecholamine, NO metabolites (NOx) and 4-hydroxy-2-nonena1 during cage-switch stressEstrogen replacement tended to suppress plasma norepinephrine elevation induced by the stress in ovariectomized rat. Additionally, the NO synthase (NOS) inhibitor decreased this effect of estrogen. By Western blotting analysis, estrogen replacement enhanced basal endothelial NOS expression in mesenteries. Furthermore, estrogen replacement attenuated a basal level of plasma 4-hydroxy-2-nonenal, a marker for oxidative stress, and tended to enhance plasma NOx level.Our results suggest that est … More rogen attenuates the stress-induced pressor response by suppressing oxidative stress and enhancing NO bioavailability(3) Effects of estrogen replacement on neuronal activation induced by cage-switch stress in ovariectomized rat brainThe stress-induced elevations in c-Fos expressions, a marker for neuronal activation, were detected in the following brain areas : lateral septal nucleus, paraventricular hypothalamic nucleus (PVN), dorsomedial hypothalamic nucleus (DMD), arcuate hypothalamic nucleus, paraventricular thalamic nucleus, and locus coeruleus (LC). Estrogen replacement suppressed stress-induced c-Fos expressions in PVN, DMD and LC. In addition, estrogen replacement enhanced the activation of NO-producing neurons in PVN. These findings suggest that estrogen replacement attenuates neuronal activation in these brain areas, at least partly by NO.These results suggest that estrogen replacement suppresses cardiovascular responses to psychological stress at least partly through its anti-oxidative effect on the periphery and through its inhibitory effect on neuronal activation in the brain of ovariectomized rats. Less

（1）用笼子中的氧化一氧化氮在笼子中刺激了雄性大鼠的去甲肾上腺素，但在女性大鼠中没有性别差异。通过一氧化氮对心理压力的反应（NO）。通过Western印迹分析，雌激素在Mesparog中增强了基底式的NOS NOS Express，EN替换减弱了基础水平的等离子4-羟基-2-非纳尔，这是氧化应激的标志...通过抑制氧化应激并增强没有生物可挥发性（3）疏远对卵巢切除率诱导的神经元激活的影响（3）对卵巢切除型大鼠的神经元激活的影响（3），更多的Rogen TE TES TES TES TES TES TES TES TES TES iid iid的压力反应（3）对于神经元激活，在以下大脑区域中检测到：侧隔核，核US（PVN），背侧下丘脑核（DMD），弧形下丘脑核，旁膜片核，副核核，丘脑核核和基因座（LC）。诱导的C-FOS表达在PVN，DMD和LC中。在外围以及通过其对卵巢大鼠大脑神经元激活的抑制作用。