Effects of fat and protein on cholesterol metabolism at the time of estrogen lack

雌激素缺乏时脂肪和蛋白质对胆固醇代谢的影响

• 批准号: 16580101
• 负责人: EBIHARA Kiyoshi
• 金额: $ 2.37万
• 依托单位: Ehime University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16580101/
• 关键词: Dietary fat; Dietary protein; Estrogen deficiency; Plasma cholesterol; Bile acid; CYP7A1; LDL-receptor; Ovariectomized rat; 血漿コレステロール濃度; 肝臓mRNA発現量; CYP7A1活性; アミノ酸組成; 脂肪酸; LDL受容体; CYP7A1; mRNA発現量

The effects of dietary fat and protein on the ovarian hormone deficiency-associated increase in plasma cholesterol concentration were studied in ovariectomized 6-month-old female rats. Oleic acid-rich safflower oil, linoleic acid-rich safflower oil, perilla oil, fish oil, hydrogenated palm oil, milk fat were used as dietary fat source. Casein, rice protein, soybean protein, potato protein, wheat protein and cod protein were used as dietary protein source. Ovariectomized rats were fed the diets based on AIN93G containing each test fat or protein for 28 days.The plasma cholesterol concentration of the fish oil group was significantly lower than those of other groups, and that of the perilla oil group was a tendency lower than those of other groups. α-linoelenic acid is changed into EPA and DHA in a body, which suggests that the plasma cholesterol concentration lowering effect of fish and perilla oil might depend on EPA and DHA. The level of LDL-receptor mRNA was a tendency lower in fish … More oil and perilla oil groups than in other groups. The level of CYP7A1 mRNA was not affected by dietary fat source. The fecal excretion of bile acid was higher in fish oil, hydrogenated palm oil and milk fat groups than in other groups. However, the increased fecal excretion of bile acid did not necessarily lead to the reduction of plasma cholesterol.The plasma cholesterol concentrations of potato protein, soybean protein and cod protein groups were significantly lower than that of casein group, but those of rice protein and wheat protein were not. I has been suggested that the higher plasma cholesterol concentration of casein might depend on the higher methionine contnt, lysine/arginine ratio and methionine/glycine ratio compared other dietary protein. The content of methionine was lower in wheat protein, rice protein, soybean protein and potato protein than that in casein, however was not significant difference between wheat protein, rice protein, soybean protein and potato protein. The content of methionine, lysine/arginine ratio and methionine/glycine ratio of cod protein was lower than those of casein. The levels of LDL-receptor mRNA and CYP7A1 were not affected by dietary protein. It was clarified that the plasma cholesterol concentration was affected by the quality of the protein, but a further examination is necessary on the relation to the amino acid composition and hypocholesterolemic effect of dietary protein. Less

在富含油酸的红花油、富含亚油酸的红花油、紫苏油、鱼油中研究了膳食脂肪和蛋白质对卵巢激素缺乏相关的血浆胆固醇浓度升高的影响。 ，氢化棕榈油、乳脂作为膳食脂肪源，大米蛋白、大豆蛋白、马铃薯蛋白、小麦蛋白和鳕鱼蛋白作为膳食蛋白源。大鼠以含有各试验脂肪或蛋白质的AIN93G为基础的饮食喂养28天。鱼油组血浆胆固醇浓度显着低于其他组，紫苏油组有低于对照组的趋势。 α-亚麻酸在体内转化为EPA和DHA，这表明鱼和紫苏油降低血浆胆固醇浓度的作用可能依赖于EPA和DHA的LDL受体mRNA水平。鱼油和紫苏油组的 CYP7A1 mRNA 水平不受膳食脂肪来源的影响 鱼油、氢化棕榈油和乳脂组的粪便胆汁酸排泄量高于其他组。然而，粪便中胆汁酸排泄量的增加并不一定导致血浆胆固醇的降低。马铃薯蛋白组、大豆蛋白组和鳕鱼蛋白组的血浆胆固醇浓度显着低于酪蛋白组，但马铃薯蛋白组、大豆蛋白组和鳕鱼蛋白组的血浆胆固醇浓度显着低于酪蛋白组。我认为，与其他膳食蛋白质相比，酪蛋白的血浆胆固醇浓度较高可能取决于较高的蛋氨酸含量、赖氨酸/精氨酸比率和蛋氨酸/甘氨酸比率。小麦蛋白、大米蛋白、大豆蛋白和马铃薯蛋白含量高于酪蛋白，而小麦蛋白、大米蛋白、大豆蛋白和马铃薯蛋白含量差异不显着。鳕鱼蛋白的赖氨酸/精氨酸比率和蛋氨酸/甘氨酸比率低于酪蛋白。LDL受体mRNA和CYP7A1的水平不受膳食蛋白质的影响。明确了血浆胆固醇浓度受饲料质量的影响。蛋白质，但需要进一步研究膳食蛋白质的氨基酸组成和降胆固醇作用的关系。