Physiological functional activities of polyphenols and influences of this polyphenol on intestinal flora.

多酚的生理功能活性及其对肠道菌群的影响。

基本信息

批准号：
09660144
负责人：
KIMURA Shuichi
金额：
$ 2.18万
依托单位：
Showa womens University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C).
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

项目摘要

(1) Examination of estrogn-like activiy for the polyphenols of the edible wild plants.Antioxidative activities of polyphenols of the edible wild plants were examined, and it was found that a strong antioxidative axtivities were admitted in two plants.In this study, the estrogen-like activity of these wild plants, okahijiki (Solosoa Komarovi Iijin ; SK) and suberihiyu(Porturaca Oleracea ; PO), were examined.Rat which removed the ovary (OVX-rat) was used as the model experimental animals for osteoporosis syndrome, and the recovery effects of the polyphenols of these two wild plants to the osteopolosis model animals were determined. In this experiment calcium and other trace elemenal contents of bone were analyzed by ICP method, and also the resistant activity for bone fracturation, mineral density of bone, alkarine phsphatase in blood were examined.Results : The contents of calcium and other trace elements of SK-supplemented rats showed the same levels of OVX-rat. It measn SK has noeffect. However, PO-supplemeted rats showed principally same level of sham-control rat. Moreover, PO-supplemented rat shows few decrease of alkarine phosphatase, high mineral density and high resistance to fractration..These facts suggest that PO has possively some estrogenic substance.(2) Influences of polyphenol on intestinal nfloraIt is thought that administering polyphenol has an some effective influence to the intestinal flora, However, concrete proof is a little. So, in this studies, the influence of polyohenol on the intestinal flora, particularly to lactic acid bacterium and bifidus bacterium which is useful bacterium were eamined with in vitro and in vivo.Results : In both experiments with in vitro and in vivo, polyphenol has supressive effect on the growth of the gram-negative bacterium. However, such an inhibitory activity dose not appear in lactic acid bacterium and bifidus bacterium.These facts suggest that polyphenol may help a good balance among intestinal bacterial flora.

(1)食用野生植物多酚的类雌激素活性检测。对食用野生植物多酚的抗氧化活性进行检测，发现两种植物具有较强的抗氧化活性。这些野生植物 okahijiki (Solosoa Komarovi Iijin ; SK) 和 suberihiyu (Porturaca Oleracea ; ) 具有类雌激素活性。以摘除卵巢的大鼠(OVX-rat)作为骨质疏松症模型实验动物，测定这两种野生植物多酚对骨质疏松模型动物的恢复作用。本实验采用ICP法分析骨中钙等微量元素含量，并检测其抗骨折活性、骨矿物质密度、血液中碱性磷酸酶。结果：SK中钙等微量元素的含量-补充的大鼠显示出相同水平的OVX-大鼠。这意味着SK没有效果。然而，补充 PO 的大鼠表现出与假手术对照大鼠基本相同的水平。此外，补充PO的大鼠碱性磷酸酶下降很少，矿物质密度高，抗碎裂能力强。这些事实表明PO可能含有一定的雌激素物质。 (2)多酚对肠道菌群的影响人们认为，给予多酚对肠道菌群有一定的影响。对肠道菌群有一定的有效影响，但具体证据还很少。因此，本研究通过体外和体内研究了多酚对肠道菌群的影响，特别是对有益菌乳酸菌和双歧杆菌的影响。结果：在体外和体内实验中，多酚均具有良好的效果。对革兰氏阴性菌的生长有抑制作用。然而，这种抑制活性在乳酸菌和双歧杆菌中并未出现。这些事实表明多酚可能有助于肠道菌群的良好平衡。